You searched for: Electrophoresis Reagents
Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
CYTAG™ CGH labelling kits
Supplier: Enzo Life Sciences
The CYTAG™ CGH labeling kit produces high quality data for comparative genomic hybridisation (aCGH) using as little as 0,25 µg of genomic DNA, without a need for pre-amplification.
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Normal cell line blots, human
Supplier: G-Biosciences
Ready to screen, normal tissue human cell line blots from epithelial, endothelial, skin, and muscle tissue.
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Alkaline Phosphatase chromogen (BCIP/TNBT), Abcam
Supplier: Abcam
This product is a liquid substrate for alkaline phosphatase (AP), and is especially useful for the detection of AP-conjugated antibodies.
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Visualizer and Visualizer EC Western Blot Detection Kits
Supplier: Merck
The Visualizer Western Blot Detection kit is designed for the sensitive detection of proteins in chemiluminescent Western blots.
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Chemicon® ReBlot Western Blot Recycling Kit
Supplier: Merck
Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.
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Mouse single tissue blot, single species
Supplier: G-Biosciences
This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.
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Rat single tissue blot, single species
Supplier: G-Biosciences
This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.
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Heart tissue region blots, single species
Supplier: G-Biosciences
These are ready to screen, normal tissue, heart tissue region blots from human and pig. The heart tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult pig and adult human hearts.
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Brain tissue region blots, single species
Supplier: G-Biosciences
G-Biosciences offers ready-to-screen, normal tissue, brain tissue region blots. The brain tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse, rat, human, and primate brains.
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Decontamination bags, activated charcoal
Supplier: Biotium
Activated charcoal retains ethidium bromide, GelRed™ and GelGreen™ efficiently in a convenient bag for decontamination of used gel staining solutions. Each bag has a total capacity of at least 10 mg ethidium bromide.
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Chemicon® ReBlot Plus Mild Antibody Stripping Solution, 10X
Supplier: Merck
Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.
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Caspase-3 substrates, NucView®
Supplier: Biotium
NucView® caspase-3 substrates are fluorescent probes that allow for the real-time detection of apoptosis by monitoring caspase-3/7 activity in intact cells using confocal microscopy, flow cytometry or microplate readers.
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Transfer pads for western blotting, Swift™
Supplier: G-Biosciences
SWIFT™ transfer pads are treated with a proprietary electrolyte buffer to enhance Western blot transfer efficiency.
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Botanical garden blot
Supplier: G-Biosciences
G-Biosciences' Botanical Garden Blot is prepared using total protein extracted from a variety of 5 to 10 day old, whole plant tissues.
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Chemiluminescent HRP Substrate (membrane), Abcam
Supplier: Abcam
A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.
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Western blots, pre-made, human single tissue single species
Supplier: G-Biosciences
Ready-to-screen, human, single tissue blots, from both normal and tumour tissues. G-Biosciences pre-made blots are prepared with the appropriate GenLysate™ and 50 μg protein is loaded onto a 4 to 20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved, they are transferred to a PVDF membrane, using our Western transfer buffer.
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NPP ELISA alkaline phosphatase substrate
Supplier: Rockland Immunochemicals
NPP ELISA alcaline phosphatase substrate is a chromogenic substrate used in immunosorbent assays.
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Luminata™ Chemiluminescent ELISA HRP Substrates
Supplier: Merck
Chemiluminescent HRP detection reagent for use on immunoblots, Western blotting, dot/slot blotting.
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Rapid DAB Detection System, Abcam
Supplier: Abcam
Rapid DAB Detection System (ab290116) is a powerful Intensification system that significantly enhances chromogenic signals.
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Multiple tissue blot, single species
Supplier: G-Biosciences
These multiple tissue, single species blots permit researchers to visualise the tissue distribution of their protein in a particular species. These have been successfully used for the analysis of a wide variety of proteins. Each blot has multiple tissue types from either human, mouse or rat species.
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DAB Substrate Kit, Abcam
Supplier: Abcam
DAB substrate kit is used for immunohistochemistry (IHC) staining using HRP/peroxidase enzyme for detection. - Cited in over 375 publications - Stable for up to 6 hours after mixing - Produces crisp brown stain, alcohol-insoluble - Convenient two-component liquid format - Refrigerator-stable for extended usability
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ECL Substrate Kit (Ultra High Sensitivity), Abcam
Supplier: Abcam
Ultra High Sensitivity ECL Substrate Kit ab133409 is a horseradish peroxidase (HRP) substrate specially developed for Western blotting detection of very low abundance proteins with 1.
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Antibody Diluent (HAMA Blocker) for ELISA, Abcam
Supplier: Abcam
Antibody Diluent (HAMA Blocker) for ELISA AB318129 is used for sandwich antibody pair based assays that are susceptible to heterophilic antibody interference.
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Western blot stripping buffer, Western ReProbe™
Supplier: G-Biosciences
Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse Western blots.
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Western ReProbe™ PLUS
Supplier: G-Biosciences
Western ReProbe™ PLUS allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies.
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NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen, Abcam
Supplier: Abcam
NBT / BCIP chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in immunoblotting.
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Chemiluminescent detection reagents, Immobilon® Western HRP substrates
Supplier: Merck
Immobilon® Western HRP substrates are compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents.
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Chemicon® ReBlot Plus Strong Antibody Stripping Solution, 10X
Supplier: Merck
Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.
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Diluent for western blotting, Swift™ Western Diluent
Supplier: G-Biosciences
The Swift™ Western Diluent is a solution that reduces the blocking and antibody incubations on Western blot membranes to <60 minutes. Swift™ Western Diluent generates comparable results to traditional Western blotting procedures and other commercial “fast” Western blotting kits.
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Antibody Diluent, Abcam
Supplier: Abcam
Antibody diluent (pH 7 - 7.8) is used to dilute primary antibodies to their working concentrations for use in immunohistochemistry (IHC). - Cited in over 15 publications - Reduces background and non-specific binding - Extends antibody stability up to 24 months at 2-8°C - Tris-buffered with stabilizing protein, wetting agent, antimicrobial agent, and green dye