You searched for: Electrophoresis Reagents
Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
Ethidium bromide adsorber for decontamination of ethidium bromide from staining solutions, Sigma-Aldrich®
Supplier: Merck
Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels.
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VWR®, Destaining bags
Supplier: VWR Chemicals
Destaining bags are designed to safely remove ethidium bromide from solution during overnight treatment. Each bag extracts up to 5 mg of ethidium bromide from solution, thus minimising the exposure of research personnel to this toxic material. The special absorbent mixture retains dye molecules in a convenient bag for removal and incineration.
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Nucleic acid and protein destain, Silver D-Stain™
Supplier: G-Biosciences
The staining of gels with silver stains often results in over-staining or unevenly stained gels. Silver D-Stain™ eliminates the need to repeat the electrophoresis.
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VWR® Fixing Solution for Isoelectric Focusing in Polyacrylamide Gel, Reag. Ph. Eur. 1138700
Supplier: VWR Chemicals
VWR®, Fixing solution for isoelectric focusing in polyacrylamide ge
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VWR® VisiGlo™ HRP Chemiluminescent Substrate Kits
Supplier: VWR Chemicals
VisiGlo™ and Visiglo Plus™ HRP chemiluminescent substrates are luminol-based chemiluminescent substrates designed for rapid and sensitive detection of peroxidase-labelled conjugates. Sensitivity ranges from picogram levels, captured by VisiGlo™ HRP chemiluminescent substrate, down to femtogram levels, exhibited by VisiGlo™ Prime and VisiGlo™ Select. VisiGlo™ Select displays the highest sensitivity, ideal for detecting low abundance proteins. The signal generated by substrate incubation is sustained between 8 to 10 hours allowing you to perform multiple exposures over an extended period of time.
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Optiblot SDS-PAGE Sample Preparation Kit, Abcam
Supplier: Abcam
The Optiblot SDS-PAGE Sample Preparation Kit (ab133414) provides a means to quickly concentrate protein samples, and separate them from buffers that interfere with electrophoresis.
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Western blot assay kits, ScanLater™
Supplier: Molecular Devices
The ScanLater™ Western Blot Assay Kits are a time-resolved fluorescence (TRF)-based assay, optimal for quantitating as little as femtogram protein samples.
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ECL Substrate Kit (High Sensitivity), Abcam
Supplier: Abcam
High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band.
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Chemiluminescent acridinium esters
Supplier: Enzo Life Sciences
Chemiluminescent acridinium esters are commonly used to label antibodies, proteins, nucleic acids, and some peptides, and are frequently used as detection reagents in immunoassays.
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Eye tissue region blot, single species
Supplier: G-Biosciences
These are ready to screen, normal tissue, eye tissue region blots from rat and rabbit. The eye tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult rat and rabbit eyes.
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Human cancer cell line blot
Supplier: G-Biosciences
Ready to screen human cancer cell line blots.
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ECL Western Blotting Substrate Kit, Abcam
Supplier: Abcam
The ECL Western Blotting Substrate is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots.
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Chemicon® ReBlot Plus Kits
Supplier: Merck
Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electrophoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.
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Rabbit specific IHC polymer detection kit HRP/DAB, Abcam
Supplier: Abcam
Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is a sensitive immunoenzymatic system for detecting antibodies bound to antigens within tissue sections by light microscopy.
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Luminata™ Western HRP Substrates Samples for Antibodies of All Affinities
Supplier: Merck
Western blotting, binding assays, amino acid analysis, N-terminal protein sequencing, dot/slot blotting, glycoprotein visualisation, lipopolysaccharide analysis.
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Nitrotyrosine BSA for immunoblotting experiments
Supplier: Cayman Chemical
This Nitrotyrosine BSA can be used as a positive control for immunoblotting experiments using Nitrotyrosine Polyclonal Antibody and Nitrotyrosine Monoclonal Antibody.
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Western blots, pre-made, mouse subcellular fraction specific blot
Supplier: G-Biosciences
The subcellular fractions from liver tissue are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse livers.
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Luminata™ Chemiluminescent ELISA HRP Substrates
Supplier: Merck
Chemiluminescent HRP detection reagent for use on immunoblots, Western blotting, dot/slot blotting.
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Rapid DAB Detection System, Abcam
Supplier: Abcam
Rapid DAB Detection System (ab290116) is a powerful Intensification system that significantly enhances chromogenic signals.
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DAB Substrate Kit, Abcam
Supplier: Abcam
DAB substrate kit is used for immunohistochemistry (IHC) staining using HRP/peroxidase enzyme for detection. - Cited in over 375 publications - Stable for up to 6 hours after mixing - Produces crisp brown stain, alcohol-insoluble - Convenient two-component liquid format - Refrigerator-stable for extended usability
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ECL Substrate Kit (Ultra High Sensitivity), Abcam
Supplier: Abcam
Ultra High Sensitivity ECL Substrate Kit ab133409 is a horseradish peroxidase (HRP) substrate specially developed for Western blotting detection of very low abundance proteins with 1.
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Western blot stripping buffer, Western ReProbe™
Supplier: G-Biosciences
Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse Western blots.
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Antibody Diluent (HAMA Blocker) for ELISA, Abcam
Supplier: Abcam
Antibody Diluent (HAMA Blocker) for ELISA AB318129 is used for sandwich antibody pair based assays that are susceptible to heterophilic antibody interference.
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Western ReProbe™ PLUS
Supplier: G-Biosciences
Western ReProbe™ PLUS allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies.
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NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen, Abcam
Supplier: Abcam
NBT / BCIP chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in immunoblotting.
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Multiple tissue blot, single species
Supplier: G-Biosciences
These multiple tissue, single species blots permit researchers to visualise the tissue distribution of their protein in a particular species. These have been successfully used for the analysis of a wide variety of proteins. Each blot has multiple tissue types from either human, mouse or rat species.
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Chemiluminescent detection reagents, Luminata™ Western HRP substrates
Supplier: Merck
Luminata™ Western HRP substrates are a family of premixed, ready to use chemiluminescent reagents for the detection of HRP-based Westerns.
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ECL Substrate Kit (Very High Sensitivity), Abcam
Supplier: Abcam
Very High Sensitivity ECL Substrate Kit () ab133408 uses a new horseradish peroxidase (HRP) substrate specially developed to take advantage of the large linear dynamic range provided by CCD imaging.
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Normal cell line blots, human
Supplier: G-Biosciences
Ready to screen, normal tissue human cell line blots from epithelial, endothelial, skin, and muscle tissue.
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IHC chromogen substrate (AEC, stable), HIGHDEF®
Supplier: Enzo Life Sciences
HIGHDEF® red IHC chromogen can be used in conjunction with peroxidase-based immunostaining systems.