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Protein Purification Reagents

Protein Purification Reagents

Protein purification reagents are for use in isolating one or more proteins from a complex mixture, usually cells, tissues, or whole organisms. This process is vital for function and structure characterization, as well as identifying interactions of the protein of interest. Important during the many steps, the protein purification reagents assist with cell lysis, separating the soluble protein components from cell debris, and finally separating the protein of interest from product- and process-related impurities.

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Recombinant protein A/G, alkaline phosphatase conjugated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Alkaline Phosphatase Conjugated Protein A/G is useful to probe and detect antibodies, especially IgG isotypes, of many different species hosts in Western blotting, ELISA, IHC and other immunoassay protocols.

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Recombinant protein L, biotinylated, Pierce™

Recombinant protein L, biotinylated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Biotinylated Recombinant Protein L can be used to probe and detect mouse and human antibodies containing kappa light chains in Western blotting, ELISA, IHC and other immunoassay protocols.

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Pegylation reagents, Methyl-PEG-NHS-Esters, Pierce™

Pegylation reagents, Methyl-PEG-NHS-Esters, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ MS(PEG)n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)12 is a three-branched, polyethylene glycol compound (3× 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.

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Pierce™ Recombinant Protein G, Biotinylated

Pierce™ Recombinant Protein G, Biotinylated

Supplier: Thermo Fisher Scientific

Biotinylated recombinant Protein G can be used to probe and detect mouse and human antibodies, especially IgG isotypes, in western blotting, ELISA, IHC and other immunoassay protocols.

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Recombinant protein A/G, peroxidase conjugated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Peroxidase Conjugated Protein A/G is useful to probe and detect antibodies, especially IgG isotypes, of many different species hosts in Western blotting, ELISA, IHC and other immunoassay protocols.

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PEGylated N-succinimidyl S-acetylthioacetate, SAT(PEG)4, Pierce™

PEGylated N-succinimidyl S-acetylthioacetate, SAT(PEG)4, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ SAT(PEG)₄ (PEGylated N-succinimidyl S-acetylthioacetate) is a sulphhydryl-addition reagent for covalent modification of primary amines.

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Protein stabilising cocktail, Pierce™

Protein stabilising cocktail, Pierce™

Supplier: Thermo Fisher Scientific

The Protein Stabilising Cocktail is a versatile stabilising solution that increases the shelf-life of purified or partially purified proteins during routine storage.

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Inclusion body solubilisation reagent, Pierce™

Inclusion body solubilisation reagent, Pierce™

Supplier: Thermo Fisher Scientific

Inclusion Body Solubilisation Reagent is designed to retrieve expressed protein in soluble form after lysis and extraction with reagents.

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Recombinant protein G, peroxidase conjugated, Calbiochem®

Supplier: Merck Millipore (Oncogene)

Purified recombinant Protein G conjugated to peroxidase can be used in direct immunoassay.

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HCCA matrix

Supplier: BRUKER DALTRONIK

Pre-portioned HCCA matrix provides easy and convenient preparation of HCCA matrix solutions.

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BTSNM support materials, beads, 0,4 g with buffer

Supplier: G-Biosciences

Beads are made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.

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Recombinant streptavidin

Recombinant streptavidin

Supplier: G-Biosciences

Recombinant streptavidin from Streptomyces avidinii, produced in E.coli.

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Coupling catalyst, GlycoLink™

Coupling catalyst, GlycoLink™

Supplier: Thermo Fisher Scientific

An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.

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Pegylation reagent, CT(PEG)₁₂ carboxy-PEG-thiol compound

Pegylation reagent, CT(PEG)₁₂ carboxy-PEG-thiol compound

Supplier: Thermo Fisher Scientific

Carboxy-PEG12-thiol, is a carboxyl- and sulphhydryl-terminated compound that contains a 12-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

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Mass spectrometry reagents, MALDI matrices, single-use

Mass spectrometry reagents, MALDI matrices, single-use

Supplier: Thermo Fisher Scientific

MALDI Matrices are highly purified, recrystallised reagents packaged in convenient single-use tubes sufficient for analysing up to 96 samples.

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Pegylation reagents, methyl-PEG-amine compounds

Pegylation reagents, methyl-PEG-amine compounds

Supplier: Thermo Fisher Scientific

The methyl-PEGn-amine (MA[PEG]n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.

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