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Electrophoresis Buffers

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

In-situ hybridisation buffer for HPV probes

Supplier: ENZO LIFE SCIENCES

In situ hybridisation buffer for HPV probes (ready-to use) provides a high quality hybridisation medium for In situ hybridisation analyses using either DNA or oligonucleotide probes labeled with biotin-, or fluorescein-modified nucleotides.

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Protein blocking buffers, Superior™

Protein blocking buffers, Superior™

Supplier: G-Biosciences

Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.

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Blocking buffers, Protein-Free™

Supplier: G-Biosciences

Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.

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SSC buffer, 20X concentrate, pH 7,0

Supplier: Rockland Immunochemicals

Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.

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Buffer, PBS (10X, Dulbecco's) powder pack

Buffer, PBS (10X, Dulbecco's) powder pack

Supplier: PanReac AppliChem

Powder mixture to prepare PBS buffer (10X) according to Dulbecco.

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Buffer, TBS solution 10X concentrate (TRIS buffered saline)

Supplier: G-Biosciences

The TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.

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WB blocking buffer kit, TrueBlack®

Supplier: Biotium

The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).

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Buffer, TBS solution 10X concentrate (TRIS buffered saline) with phenol red, pH 7,4

Supplier: Cayman Chemical

250 mM Tris-base, pH 7,4, 1,37 M sodium chloride, containing 27 mM KCl, 0,4 mM phenol red.

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Protein blocking agent, BLOT-QuickBlocker™

Protein blocking agent, BLOT-QuickBlocker™

Supplier: G-Biosciences

BLOT-QuickBlocker™ is a modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background.

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SSPE Buffer, 20X concentrate, pH 7,4

Supplier: Rockland Immunochemicals

20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: G-Biosciences

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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Dry buffer packs, JAW™

Supplier: G-Biosciences

Premixed buffer powders that simply require adding water to generate a ready-to-use buffer.

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TPE buffer, 10X tris-phosphate-EDTA, pH 8

TPE buffer, 10X tris-phosphate-EDTA, pH 8

Supplier: Rockland Immunochemicals

Running Buffer, Tris-phosphate-EDTA 10X (TPE) (0,9 M tris-phosphate 0,01 M EDTA pH 8,0)

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Buffer, PBS tablets

Buffer, PBS tablets

Supplier: PanReac AppliChem

PBS Tablets for preparation of 200 ml phosphate buffered saline (PBS). Composition of 140 mM sodium chloride, 2,7 mM potassium chloride and 10 mM phosphate.

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Buffer, TBS solution 10X concentrate (TRIS buffered saline) pH 7,4

Supplier: Cayman Chemical

Formulation: Concentrated TBS (250 mM Tris-base; pH 7,4; 1,37 M sodium chloride and 27 mM KCl).

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In situ hybridisation wash reagent

In situ hybridisation wash reagent

Supplier: ENZO LIFE SCIENCES

Hybridisation wash reagent provides a high quality hybridisation wash solution for in situ hybridisation analyses using DNA probes labeled with biotin, digoxigenin or fluorescein.

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Blocking buffers

Blocking buffers

Supplier: Rockland Immunochemicals

A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.

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PBS and TBS buffers

PBS and TBS buffers

Supplier: Rockland Immunochemicals

Phosphate buffered and Tris buffered saline are suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.

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Blocking buffer, fish gelatin

Supplier: Biotium

Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

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TAE buffer (20X), Pierce™

TAE buffer (20X), Pierce™

Supplier: Thermo Fisher Scientific

20X TAE buffer is a space-saving stock solution that is ideal for casting and preparing Tris-Acetate EDTA running buffer used for agarose gel electrophoresis of nucleic acids.

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TAE buffer, 50X

Supplier: EDVOTEK

This 50X concentrated solution (Tris-acetate, EDTA, pH 7,8) is sufficient for making 5 L of diluted working buffer for making agarose gels and electrophoresis chamber buffer.

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: OMEGA BIO-TEK

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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North2South™ Hybridisation Buffer

North2South™ Hybridisation Buffer

Supplier: Thermo Fisher Scientific

North2South™ hybridisation buffer is an aqueous buffer which is used with the North2South™ chemiluminescent detection kit.

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Wash buffer, Pierce™

Wash buffer, Pierce™

Supplier: Thermo Fisher Scientific

These wash buffers are designed for use in dialysis, cross-linking, enzyme assays, ELISAs, immunohistochemistry, protein plate-coating, biotinylation applications.

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Pierce™ BupH™ Wash Buffers, Dry Blend Pack

Pierce™ BupH™ Wash Buffers, Dry Blend Pack

Supplier: Thermo Fisher Scientific

BupH packs are pre-blended and pre-measured dry mixtures of buffers that are easy to prepare and used in protein purification and common laboratory techniques.

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Blocking solution, BLOK™ Casein

Blocking solution, BLOK™ Casein

Supplier: G-Biosciences

BLOK™ Casein is a purified protein, used for blocking free binding sites in Western blot, ELISA plate well, immunohistochemistry and other immunochemical applications.

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Pierce™ Lane Marker Non-reducing Sample Buffer (5X)

Pierce™ Lane Marker Non-reducing Sample Buffer (5X)

Supplier: Thermo Fisher Scientific

Pierce™ Lane Marker Non-Reducing Sample Buffer is a ready-to-use 5X SDS-PAGE sample loading buffer in a non-reducing formulation, with a pink dye buffer-front marker.

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Blocker™ Blocking Buffer, BLOTTO in TBS

Blocker™ Blocking Buffer, BLOTTO in TBS

Supplier: Thermo Fisher Scientific

Blocker™ BLOTTO is a ready-to-use 5% solution of non fat powdered milk in Tris-buffered saline for Western blot, ELISA, IHC and other detection methods.

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Buffer, TBE (Tris-borate-EDTA) powder for 10X concentrated solution

Supplier: PanReac AppliChem

Powder for 10X concentrated aqueous solution (170,28 g/L). Composed of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂.

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Tris-Glycine native gel running buffer

Supplier: G-Biosciences

Running Buffer, Tris-Glycine [10X]

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