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You searched for: Protein Purification

Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

Co-immunoprecipitation (Co-IP) Kit, Pierce™

Co-immunoprecipitation (Co-IP) Kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Co-Immunoprecipitation (Co-IP) Kit provides covalent antibody immobilisation and all the components necessary to perform a properly controlled co-IP experiment without antibody interference in the final product.

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Enterokinase cleavage capture kit

Supplier: Merck Millipore (Novagen)

The Enterokinase Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins followed by rapid, affinity-based capture and removal of enterokinase. Following cleavage of the target protein, rEK is removed with >99% efficiency from the reaction by affinity capture on EKapture™ Agarose. Following capture of rEK, the EKapture Agarose is removed by spin filtration, because the same buffer conditions are used for both cleavage and capture no buffer changes are necessary.

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Pegylation reagents, methyl-PEG-amine compounds

Pegylation reagents, methyl-PEG-amine compounds

Supplier: Thermo Fisher Scientific

The methyl-PEGn-amine (MA[PEG]n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.

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Pegylation reagent, CT(PEG)₁₂ carboxy-PEG-thiol compound

Pegylation reagent, CT(PEG)₁₂ carboxy-PEG-thiol compound

Supplier: Thermo Fisher Scientific

Carboxy-PEG12-thiol, is a carboxyl- and sulphhydryl-terminated compound that contains a 12-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

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Hydrophobic interaction chromatography media, Capto™ Phenyl (high sub)

Hydrophobic interaction chromatography media, Capto™ Phenyl (high sub)

Supplier: Cytiva

Capto™ Phenyl (high sub) is used in the capture and intermediate stages of protein purification.

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Ion exchange chromatography media, Capto™ Q ImpRes

Ion exchange chromatography media, Capto™ Q ImpRes

Supplier: Cytiva

Capto™ Q ImpRes is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.

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Affinity chromatography media, IMAC Sepharose™ High Performance

Affinity chromatography media, IMAC Sepharose™ High Performance

Supplier: Cytiva

IMAC Sepharose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

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Affinity chromatography media, Dextrin Sepharose™ High Performance

Affinity chromatography media, Dextrin Sepharose™ High Performance

Supplier: Cytiva

Dextrin Sepharose™ High Performance is an affinity media for purification of recombinant proteins tagged with Maltose Binding Protein (MBP).

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Ion exchange chromatography media, Capto™ DEAE

Ion exchange chromatography media, Capto™ DEAE

Supplier: Cytiva

Capto™ DEAE is a strong anion exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.

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Affinity chromatography media, TALON® Superflow™

Affinity chromatography media, TALON® Superflow™

Supplier: Cytiva

Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant proteins by immobilised metal affinity chromatography (IMAC).

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Capto™ MMC ImpRes multimodal chromatography media, Cytiva

Capto™ MMC ImpRes multimodal chromatography media, Cytiva

Supplier: Cytiva

Capto MMC ImpRes can be used for initial or final polishing steps in MAb purification processes, as well as for polishing of antibody fragments such as domain antibodies (DAbs). The wide window of operation for conductivity and/or pH simplifies the process as conditioning after the protein A step can be omitted.

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Protein G, MP Biomedicals

Supplier: MP Biomedicals

Protein G is a monomeric protein lacking cysteine residues. Recombinant Protein G is a highly stable surface receptor that is capable of binding the Fc portion of immunoglobulins, especially IgGs from a large number of species.

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Affinity chromatography media, Capto™ Blue

Affinity chromatography media, Capto™ Blue

Supplier: Cytiva

Capto™ Blue is an affinity medium for the capture of albumin, purification of enzymes, and recombinant proteins at laboratory and process scales.

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Multimodal chromatography media, Capto™ Core 700

Multimodal chromatography media, Capto™ Core 700

Supplier: Cytiva

Capto™ Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules (molecular weight >700000).

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Gel filtration media, Sephadex™ G-25

Gel filtration media, Sephadex™ G-25

Supplier: Cytiva

Sephadex™ G-25 is well established gel filtration medium for desalting and buffer exchange in industrial applications.

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Ion exchange chromatography media, SOURCE™ 30Q

Ion exchange chromatography media, SOURCE™ 30Q

Supplier: Cytiva

SOURCE™ 30Q is a synthetic high performance, preparative, chromatography medium, based on a 30 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.

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Sepharose™ CL-4B Phenyl Hydrophobic Interaction Chromatography Media

Sepharose™ CL-4B Phenyl Hydrophobic Interaction Chromatography Media

Supplier: Cytiva

Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.

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Hydrophobic interaction chromatography media, Capto™ SP ImpRes

Hydrophobic interaction chromatography media, Capto™ SP ImpRes

Supplier: Cytiva

Capto™ SP ImpRes is a strong cation exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.

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Affinity chromatography media, Glutathione Sepharose™ 4B

Affinity chromatography media, Glutathione Sepharose™ 4B

Supplier: Cytiva

Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

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Affinity chromatography media, Glutathione Sepharose™ High Performance

Affinity chromatography media, Glutathione Sepharose™ High Performance

Supplier: Cytiva

Glutathione Sepharose High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

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Protein purification system, ÄKTA™ start

Protein purification system, ÄKTA™ start

Supplier: Cytiva

ÄKTA start is an easy to use chromatography system that automates manual purification procedures using, for example, HiTrap columns. It offers an entry level chromatography solution for the preparative purification of proteins at lab scale.

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Sepharose Chromatography Resins, Strep-Tactin® XT

Sepharose Chromatography Resins, Strep-Tactin® XT

Supplier: Cytiva

A chromatography resin for purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag. These proteins bind very specifically to the immobilised Strep-Tactin XT ligand giving highly pure target protein.

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Gelatin-Sepharose® 4B

Gelatin-Sepharose® 4B

Supplier: Cytiva

Gelatin Sepharose® 4B affinity media with immobilised gelatin is designed for the purification or depletion of fibronectin as gelatin binds to fibronectin with high specificity. Gelatin Sepharose® 4B is bovine gelatin coupled to Sepharose® 4B by the cyanogen bromide method.

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Multimodal chromatography media, Capto™ MMC

Multimodal chromatography media, Capto™ MMC

Supplier: Cytiva

Capto™ MMC is a multimodal cation exchanger with a different selectivity, compared with traditional ion exchangers.

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Affinity coupling media, CNBr-Activated Sepharose™ 4B

Affinity coupling media, CNBr-Activated Sepharose™ 4B

Supplier: Cytiva

CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.

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Affinity chromatography media, Streptavidin Sepharose™ High Performance

Affinity chromatography media, Streptavidin Sepharose™ High Performance

Supplier: Cytiva

Streptavidin Sepharose™ High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance.

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Affinity chromatography media, HiTrap™ Capto™ Lentil Lectin

Affinity chromatography media, HiTrap™ Capto™ Lentil Lectin

Supplier: Cytiva

Capto™ Lentil Lectin is an affinity chromatography resin for purification of glycoproteins and other molecules containing carbohydrates such as α-D-mannose and α-D-glucose or sterically related residues.

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Affinity chromatography media, Ni Sepharose™ High Performance

Affinity chromatography media, Ni Sepharose™ High Performance

Supplier: Cytiva

Ni Sepharose™ High Performance affinity media for high resolution, high capacity purification of histidine-tagged proteins by immobilised metal ion affinity chromatography (IMAC).

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Protease affinity chromatography media, PreScission™, Cytiva

Protease affinity chromatography media, PreScission™, Cytiva

Supplier: Cytiva

Enables the low-temperature cleavage of fusion proteins containing the PreScission Protease recognition sequence.

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Gel filtration media, Sephacryl™ High Resolution

Gel filtration media, Sephacryl™ High Resolution

Supplier: Cytiva

Sephacryl™ High Resolution (HR) gel filtration media allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scale.

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