Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.
Sepharose™ CL-2B Gel Filtration Base Matrix
Supplier: Cytiva
Sepharose CL-2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
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Affinity coupling media, NHS-Activated Sepharose™ 4 Fast Flow
Supplier: Cytiva
NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.
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Affinity chromatography media, HiTrap™ Capto™ Lentil Lectin
Supplier: Cytiva
Capto™ Lentil Lectin is an affinity chromatography resin for purification of glycoproteins and other molecules containing carbohydrates such as α-D-mannose and α-D-glucose or sterically related residues.
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Protease affinity chromatography media, PreScission™, Cytiva
Supplier: Cytiva
Enables the low-temperature cleavage of fusion proteins containing the PreScission Protease recognition sequence.
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Affinity chromatography media, IMAC Sepharose™ 6 Fast Flow
Supplier: Cytiva
IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.
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Gel filtration media, Sephadex™ G-15
Supplier: Cytiva
Sephadex™ G-15 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1500 molecular weight.
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Affinity chromatography media, IgG Sepharose™ 6 Fast Flow
Supplier: Cytiva
lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose 6 Fast Flow matrix, with human IgG covalently coupled to it.
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Ion exchange chromatography media, SP Sepharose™ High Performance
Supplier: Cytiva
SP Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with sulphopropyl (SP) strong cation exchange groups.
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Ion exchange chromatography media, IEX Sepharose™ Fast Flow
Supplier: Cytiva
Q Sepharose Fast Flow is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades.
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Sepharose™ 4B Gel Filtration Base Matrix
Supplier: Cytiva
Sepharose™ 4B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
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Gel filtration media, Sephadex™ G-50
Supplier: Cytiva
Sephadex™ G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.
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Sepharose™ 4 FF Octyl Fast Flow Hydrophobic Interaction Chromatography Media
Supplier: Cytiva
Octyl Sepharose™ 4 Fast Flow resin is based on cross-linked 4% agarose. The aliphatic ligand is immobilised to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.
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Butyl Sepharose™ High Performance Hydrophobic interaction chromatography media
Supplier: Cytiva
Butyl Sepharose™ HP media is particularly well suited for intermediate purification and polishing steps providing high resolution due to the small particle size.
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Affinity chromatography media, Blue Sepharose™ High Performance
Supplier: Cytiva
This media is suitable for affinity chromatography.
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Phenyl Sepharose™ 6 Fast Flow (High Sub) Hydrophobic Interaction Chromatography Media
Supplier: Cytiva
Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity
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Accessories for ÄKTA chromatography systems
Supplier: Cytiva
Tubing connector, SRTC, PEEK, I.Ø 1,5 mm, 2 ports
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Affinity chromatography media, Lentil Lectin Sepharose™ 4B
Supplier: Cytiva
Lentil lectin is a metalloprotein, containing Ca₂₊ and Mn₂₊, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars.
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Ion exchange chromatography media, ANX Sepharose™ 4 Fast Flow (High Sub)
Supplier: Cytiva
ANX Sepharose™ 4 Fast Flow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers.
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Ion exchange chromatography media, Q Sepharose™ High Performance
Supplier: Cytiva
Q Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups.
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DEAE Sephadex™ A-25 Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.
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Plasmid purification media, PlasmidSelect Xtra
Supplier: Cytiva
PlasmidSelect Xtra is a thiophilic aromatic adsorption chromatography medium with a selectivity that allows generic purification of supercoiled covalently closed circular forms of plasmid DNA to be separated from open circular forms.
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Affinity chromatography media, MabSelect™
Supplier: Cytiva
MabSelect™ is a protein A affinity resin designed for capturing monoclonal antibodies (mAbs) from large sample volumes.
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Affinity chromatography media, Benzamidine Sepharose™ 4 Fast Flow (High Sub)
Supplier: Cytiva
Benzamidine Sepharose™ 4 Fast Flow (High Sub) is a tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as zymogens, including urokinase and prekallikrein.
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Con A Sepharose™ 4B Affinity Chromatography Media, Cytiva
Supplier: Cytiva
Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method.
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CM Sephadex™ C-25 Ion Exchange Chromatography Media
Supplier: Cytiva
Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.
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Gel filtration media, Superdex™ 30 PG
Supplier: Cytiva
Superdex prep grade is a preparative gel filtration medium with a composite matrix of dextran and agarose
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Sepharose™ CL-6B Gel Filtration Base Matrix
Supplier: Cytiva
Sepharose™ CL-6B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.
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Hydrophobic interaction chromatography media, Butyl Sepharose™ 4 Fast Flow
Supplier: Cytiva
Butyl Sepharose 4 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.
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Concentrator
Supplier: G-Biosciences
The concentrator is a high molecular weight polymer which will not migrate across the dialysis membrane. The concentrator is placed against the dialysis membrane of the Tube-O-DIALYZER™, where it rapidly absorbs water from the sample and reduces the sample volume. The concentrator can be used with dialysis bags and other dialysis devices.
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Affinity chromatography media, MabSelect SuRe™
Supplier: Cytiva
MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.