You searched for: Protein Purification
Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.
Q Sepharose™ XL Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
Q Sepharose XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established Q Sepharose Fast Flow media.
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Sepharose™ CL-4B Octyl Hydrophobic Interaction Chromatography Media
Supplier: Cytiva
Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.
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Affinity coupling media, EAH Sepharose™ 4B
Supplier: Cytiva
EAH Sepharose™ 4B allows for the stable carbodiimide-based coupling of carboxyl groups to Sepharose® 4B via an 11-atom hydrophilic spacer arm that permits extremely stable coupling.
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Ion exchange chromatography media, SOURCE™ 30S
Supplier: Cytiva
SOURCE 30S is a synthetic high performance, preparative, chromatography medium, based on a 30 µm monosized, rigid polystyrene/divinyl benzene polymer matrix It is modified with sulphonate (S) strong cation exchange groups.
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Affinity chromatography media, Heparin Sepharose™ 6 Fast Flow
Supplier: Cytiva
Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand. Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.
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Sepharose™ 6B Gel Filtration Base Matrix
Supplier: Cytiva
Sepharose™ 6B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
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Calmodulin Sepharose™ 4B Affinity Chromatography Media, Cytiva
Supplier: Cytiva
Calmodulin Sepharose™ 4B is calmodulin immobilised by the CNBr method to Sepharose™ 4B. Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells. This protein is involved in many cellular processes such as glycogen metabolism, cytoskeletal control, neurotransmission, phosphate activity and control of NAD⁺/NADP⁺.
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Blue Sepharose™ 6 Fast Flow Affinity Chromatography Media, Cytiva
Supplier: Cytiva
Blue Sepharose™ 6 Fast Flow is cibacron blue 3G coupled to Sepharose™ 6 Fast Flow. It is well established as an adsorbent for albumin and interferon at both laboratory and process scale. The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.
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Accessories and spare parts for ÄKTApilot
Supplier: Cytiva
TC gasket, 25/4
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Protein A from Staphylococcus aureus, MP Biomedicals
Supplier: MP Biomedicals
Protein A is a recombinant Fc-binding protein from Staphylococcus aureus, Cowan strain-1 gene. The product is a carboxy truncated material containing 301 amino acid residues. The non-binding regions have been removed to minimise potenial steric interference.
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SDS-PAGE Clean Up Kit
Supplier: Cytiva
SDS-PAGE clean up kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyse due to the presence of salts or low protein concentration.
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Superose™ 6 Gel Filtration Media, Prep Grade
Supplier: Cytiva
Superose™ 6 PG for separations of proteins and other biomolecules according to size. Wide separation in two molecular weight ranges from 1000 up to 5 million.
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Biacore consumables and accessories
Supplier: Cytiva
Biacore maintenance kits are convenient kits for proper instrument maintenance.
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Ion exchange chromatography media, Capto™ S ImpAct
Supplier: Cytiva
Capto™ S ImpAct is a strong cation exchange chromatography medium (resin) developed for polishing of monoclonal antibodies (MAbs) and other biomolecules.
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SP Sepharose™ XL Ion Exchange Chromatography Media
Supplier: Cytiva
SP Sepharose™ XL is a strong cation exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.
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Ion exchange chromatography media, SOURCE™ 15Q
Supplier: Cytiva
Mono-sized 15 um rigid beads gives low back pressure and high flow rates allowing for high productivity.
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Affinity chromatography media, MabSelect SuRe™ LX
Supplier: Cytiva
MabSelect SuRe™ LX is a protein A affinity medium with very high dynamic binding capacity at extended residence times and is developed for high titer antibody processes.
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Affinity coupling media, CNBr-activated Sepharose™ 4
Supplier: Cytiva
CNBr-activated Sepharose™ 4 Fast Flow is based on the Sepharose™ Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide.
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Reversed phase chromatography media, SOURCE™ 15RPC
Supplier: Cytiva
SOURCE™ 15RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for polishing steps in industrial processes requiring high flow velocities and low back-pressure.
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Gel filtration media, Sephadex™ G-100
Supplier: Cytiva
Sephadex™ G-100 is useful for desalting and buffer exchange of very large biomolecules. Sephadex™ G-100 Superfine is designed for specific cases requiring very high resolution.
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Ion exchange chromatography media, CM Sepharose™ Fast Flow
Supplier: Cytiva
CM Sepharose™ Fast Flow is a weak cation exchanger based on the well established Sepharose™ Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
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Protein G, MP Biomedicals
Supplier: MP Biomedicals
Protein G is a monomeric protein lacking cysteine residues. Recombinant Protein G is a highly stable surface receptor that is capable of binding the Fc portion of immunoglobulins, especially IgGs from a large number of species.
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Gel filtration media, Sephadex™ G-25
Supplier: Cytiva
Sephadex™ G-25 is well established gel filtration medium for desalting and buffer exchange in industrial applications.
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Sepharose™ CL-4B Phenyl Hydrophobic Interaction Chromatography Media
Supplier: Cytiva
Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.
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Ion exchange chromatography media, SOURCE™ 30Q
Supplier: Cytiva
SOURCE™ 30Q is a synthetic high performance, preparative, chromatography medium, based on a 30 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.
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Affinity coupling media, CNBr-Activated Sepharose™ 4B
Supplier: Cytiva
CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.
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Protein G Sepharose™ 4 Fast Flow Affinity Chromatography Media
Supplier: Cytiva
Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.
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DEAE Sephadex™ A-50 Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.
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Butyl-S Sepharose™ 6 Fast Flow Hydrophobic interaction chromatography media
Supplier: Cytiva
Butyl-S Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with butyl groups via a thio-ether linkage. This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively low salt concentrations.
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Sepharose™ CL-2B Gel Filtration Base Matrix
Supplier: Cytiva
Sepharose CL-2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.