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309 results for Protein Purification

 

Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

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Affinity chromatography media, HisPur™ Ni-NTA Agarose

Affinity chromatography media, HisPur™ Ni-NTA Agarose

Supplier: Thermo Fisher Scientific

HisPur™ Ni-NTA resin is a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

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Pierce™ Antibody Purification Kits, Chicken IgY Purification Kit

Pierce™ Antibody Purification Kits, Chicken IgY Purification Kit

Supplier: Thermo Fisher Scientific

Pierce™ chicken IgY purification kit purifies chicken antibodies from hen egg yolks using a unique delipidation reagent to separate protein from lipid and a precipitation reagent to selectively isolate the IgY fraction.

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Affinity purification resin, immobilised p-Aminophenyl phosphoryl choline gel, Pierce™

Affinity purification resin, immobilised p-Aminophenyl phosphoryl choline gel, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Immobilised p-Aminophenyl Phosphoryl Choline Gel enables purification of C-reactive protein (CRP) from plasma, ascites and other biological fluids.

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Immobilised protein A, UltraLink™, Pierce™

Immobilised protein A, UltraLink™, Pierce™

Supplier: Thermo Fisher Scientific

Protein A UltraLink™ and Protein A Plus UltraLink™ resins are extremely durable beaded polyacrylamide supports to which IgG-binding Protein A has been immobilised for use in IgG purification methods. Protein A resin is used to bind and purify IgG in gravity-flow, spin-column or medium pressure chromatography methods.

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ReadiPrep™ Protein G-Agarose Resin

ReadiPrep™ Protein G-Agarose Resin

Supplier: AAT Bioquest

ReadiPrep™ Protein G-Agarose Resin is a high-quality affinity resin designed for the isolation and purification of immunoglobulin classes, subclasses, and fragments from mammalian serum and biological fluids.

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Immobilised Protein G, UltraLink™, Pierce™

Immobilised Protein G, UltraLink™, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Protein G UltraLink™ and Protein G Plus UltraLink™ resins are useful for purification of antibodies and immunoprecipitation (IP). Samples containing IgG are incubated with the resin-immobilised Protein G in a buffer that facilitates binding. After non-IgG and non-antigen components of the sample are washed from the resin, the bound IgG and antigen may be recovered by elution.

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Immobilisation kit for proteins, SulfoLink™, Pierce™

Immobilisation kit for proteins, SulfoLink™, Pierce™

Supplier: Thermo Fisher Scientific

SulfoLink™ Immobilisation Kit for Proteins is a kit containing all the reagents for preparing the protein sample for efficient immobilisation and columns containing SulfoLink™ resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.

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HisPur™ Ni-NTA superflow agarose, Pierce™

HisPur™ Ni-NTA superflow agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ HisPur™ Ni-NTA Superflow Agarose is a nitrilotriacetic acid modified Superflow 6 support charged with divalent nickel (Ni⁺²) used for FPLC purification of poly-histidine-tagged proteins.

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Monomeric avidin agarose kit, Pierce™

Monomeric avidin agarose kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Monomeric Avidin Kit enables mild affinity purification of biotinylated proteins, peptides and other molecules. Avidin monomers have a much lower biotin-binding affinity than native tetrameric avidin enabling dissociation of biotinylated molecules using mild elution conditions.

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Pierce™, NAb™ Affinity Purification Kits, Spin Kits for Antibody Purification

Pierce™, NAb™ Affinity Purification Kits, Spin Kits for Antibody Purification

Supplier: Thermo Fisher Scientific

The Pierce™ NAb™ Spin Kits are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each pre-filled microcentrifuge spin column of the immobilised protein resin enables quick purification of 100 to 1000 µg of IgG from 25 to 500 µl of serum or other sample. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used.

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Glutathione-S-Transferase, Pierce™

Glutathione-S-Transferase, Pierce™

Supplier: Thermo Fisher Scientific

A solution of glutathione-S-transferase (≥100 units/mg).

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SulfoLink™ Coupling Resin

SulfoLink™ Coupling Resin

Supplier: Thermo Fisher Scientific

SulfoLink™ coupling resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilisation of cysteine-peptides and other sulfhydryl molecules.

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Affinity chromatography resin, CDI-activated agarose, Pierce™

Affinity chromatography resin, CDI-activated agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ CDI-activated agarose is carbonyldiimidazole affinity chromatography resin, activated for covalent immobilisation of N-nucleophiles and primary amine ligands in aqueous or organic solvent conditions.

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High Select™ top14 abundant protein depletion resin, Pierce™

Supplier: Thermo Fisher Scientific

Thermo Scientific™ High Select Top14 Abundant Protein Depletion Resin decreases the abundant protein and antibody components of human plasma samples, helping prepare the samples for mass spectrometry or 1D or 2D electrophoresis.

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Classic IP kit, Pierce™

Classic IP kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Classic IP Kit uses protein A/G agarose beads, microcentrifuge spin columns and buffers to perform traditional immunoprecipitation assays.

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Pierce™ Protein A/G Coated Plate IP Kit

Pierce™ Protein A/G Coated Plate IP Kit

Supplier: Thermo Fisher Scientific

Pierce™ protein A/G coated plate IP kit uses coated protein A/G microplates to perform immunoprecipitation assays in 96-well plates without resins, beads, centrifugation or magnets.

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Immobilised GST agarose, Pierce™

Immobilised GST agarose, Pierce™

Supplier: Thermo Fisher Scientific

Immobilised GST is glutathione S-transferase from Schistosoma japonicum that has been covalently immobilised on crosslinked 6% beaded agarose.

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c-Myc Tag IP/Co-IP Kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ c-Myc Tag IP/Co-IP Kit provides a simple and fast method to study c-Myc-tagged proteins, with advantages over the traditional IP procedure using protein A/G agarose.

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Streptavidin HRP conjugates, high sensitivity, Pierce™

Streptavidin HRP conjugates, high sensitivity, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ High Sensitivity Streptavidin-HRP is a peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

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Crosslink magnetic IP/Co-IP kit, Pierce™

Crosslink magnetic IP/Co-IP kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Crosslink Magnetic IP/Co-IP Kit uses crosslinking chemistry to covalently immobilise IP antibodies onto premium-quality protein A/G magnetic beads for effective immunoprecipitation and co-immunoprecipitation.

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Immobilised jacalin agarose, Pierce™

Immobilised jacalin agarose, Pierce™

Supplier: Thermo Fisher Scientific

Jacalin is an alpha-D-galactose binding lectin extracted from jack-fruit seeds (Artocarpus integrifolia). This lectin is a glycoprotein (approx. 40 kDa) composed of four identical subunits. Jacalin binds to human IgA1 (also called serum or secretory IgA) with high specificity (i.e., it does not bind IgG or IgM). Thus, Jacalin that has been immobilised on crosslinked beaded agarose is useful for purifying human IgA from serum or colostrum samples.

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Monomeric avidin agarose, Pierce™

Monomeric avidin agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Monomeric avidin agarose is immobilised onto 4% beaded agarose for purifying biotinylated proteins, peptides and other molecules.

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Affinity purification kits, Melon™ Gel spin plate kit for IgG screening

Affinity purification kits, Melon™ Gel spin plate kit for IgG screening

Supplier: Thermo Fisher Scientific

The Melon™ Gel Spin Plate Kit for IgG Screening offers a high throughput format for quick purification and screening of antibodies.

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Immobilised TCEP disulphide reducing gel, Pierce™

Immobilised TCEP disulphide reducing gel, Pierce™

Supplier: Thermo Fisher Scientific

Immobilised TCEP disulphide Reducing Gel eliminates the need to use laborious and inefficient gel filtration methods to separate the reduced sample from the reducing agent.

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Amino acid modification reagents, sulfhydryl addition kit, Pierce™

Amino acid modification reagents, sulfhydryl addition kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Sulfhydryl Addition Kit contains all necessary components to introduce free sulfhydryl groups into any primary amine-containing molecule, purify the modified molecule and quantify the added sulfhydryl groups.

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Classic magnetic IP/Co-IP kit, Pierce™

Classic magnetic IP/Co-IP kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Classic Magnetic IP/Co-IP Kit enables highly effective and efficient immunoprecipitation (IP) and co-immunoprecipitation (co-IP) of antigens and protein-complexes using less than 10 µg of antibody and a magnetic separator.

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Hydrazide resin, UltraLink™

Hydrazide resin, UltraLink™

Supplier: Thermo Fisher Scientific

UltraLink™ Hydrazide resin is used to prepare affinity columns with antibodies or other glycolsylated proteins.

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Pierce™, CarboxyLink™ Immobilisation Kit

Pierce™, CarboxyLink™ Immobilisation Kit

Supplier: Thermo Fisher Scientific

CarboxyLink™ immobilisation kit is for covalent immobilisation of peptides or other carboxyl-containing (—COOH) molecules to a porous, beaded resin for use in affinity purification procedures.

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Maleimide-activated protein, NeutrAvidin™ Pierce™

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Maleimide Activated NeutrAvidin™ Protein is a specially-prepared form of avidin that decreases background in biotin-binding.

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Immunoprecipitation, magnetic ChIP kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Magnetic ChIP Kit provides a convenient method for efficient isolation of chromatin-bound DNA by immunoprecipitation for subsequent quantitation by PCR.

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