Cellular Assays

Supplier: Enzo Life Sciences

This kit is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry.

Supplier: Biotium

The Bacterial Viability and Gram Stain Kit contains three components: CF™488A-WGA, DAPI, and EthD-III solutions for distinguishing between gram-negative and gram-positive, as well as live versus dead bacteria.

Supplier: Enzo Life Sciences

ELS offers a large collection of superoxide dismutase products for oxidative stress research.

Supplier: Enzo Life Sciences

1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway. 2. Ubiquitinylation of proteins of interest contained in exogenously added expression culture/cell extracts or tissue lysates/extracts, followed by their immediate detection/analysis or isolation/purification for use in subsequent experiments. 3. Modification of proteins using ubiquitin derivatives or mutants for improved detection/analysis or investigation of alternative (non-proteasomal) ubiquitin related pathways in subsequent experiments. For example, biotinylated-ubiquitin (Prod. No. BML-UW8705), for sensitive detection

Supplier: Enzo Life Sciences

For the quantitative determination of hydrogen peroxide in culture supernatants and cell lysates from any species.

Supplier: Molecular Devices

The FLIPR® Calcium 4 Assay Kit provides a fast, simple and reliable fluorescence-based assay for detecting changes in intracellular calcium.

Supplier: Enzo Life Sciences

Red IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridisation systems.

Supplier: Enzo Life Sciences

LYSO-ID® Green Detection Kit is an acidic organelle-selective dye specially designed to use with RFP-expressing cell lines, as well as cells expressing blue, cyan or orange fluorescent proteins (BFPs, CFPs, OFPs).

Supplier: Biotium

Flow Cytometry Fixation/Permeabilisation Kit contains optimally formulated buffers for fixation and permeabilisation of suspension cells for immunofluorescence staining of intracellular antigens for analysis by flow cytometry. The bufffers are also available separately.

Supplier: Enzo Life Sciences

For the quantitative determination of nitrite and nitrate levels in culture supernatants, plasma, serum, saliva, and urine from any species.

Supplier: Molecular Devices

SpectraMax® Quant™ dsDNA Assay kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations.

Supplier: Molecular Devices

SpectraMax® DuoLuc™ reporter assay kit offers sensitive detection of dual luciferase reporter gene assays, which are important tools for monitoring gene expression and other cellular events.

Supplier: Molecular Devices

EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.

Supplier: Cayman Chemical

A simple, reproducible, and sensitive tool for assaying free thiol content in samples.

Supplier: Biotium

Viability PCR With PMA and PMAxx™ dyes

Supplier: Rockland Immunochemicals

These assays are non-radioactive methods for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A specific dsDNA sequence containing the NF[kappa]B response element is immobilized on the bottom of the wells in a 96-well plate. The transcription factor is detected by the addition of a specific primary antibody. A colorimetric readout at 450 nm is obtained through a horseradish peroxidase-conjugated secondary antibody.