Cellular Assays
Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.
ROS-ID™ total ROS/superoxide detection kit
Supplier: Enzo Life Sciences
The kit is designed to directly monitor real time reactive oxygen species (ROS) production and Specifically Superoxide in live cells using fluorescence microscopy or flow cytometry.
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Steady membrane staining kits, CellBrite™
Supplier: Biotium
Cell surface staining kits with fluorescent membrane dye plus enhancer for imaging live cell surface for several hours to days.
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FLUOR DE LYS® HDAC3/NCOR1 fluorometric drug discovery kit
Supplier: Enzo Life Sciences
The HDAC3/NCOR1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC3 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.
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Firefly luciferase assay kit (Lyophilised)
Supplier: Biotium
This kit is designed to measure firefly luciferase activity in transfected cell reporter assays.
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p70 S6K activity kit
Supplier: Enzo Life Sciences
The p70 S6K activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
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Calcium assay kit
Supplier: Cayman Chemical
For the determination of total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates.
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Ubiquitin activating kit
Supplier: Enzo Life Sciences
Ubiquitin Activating Kit for activation of ubiquitin for use in ubiquitinylation experiments. The kit provides the means of generating thioester linked, activated ubiquitin-E1 conjugates, utilising the first step in the ubiquitin cascade, for investigation of ubiquitin activation, subsequent ubiquitin transfer to/interaction with E2 conjugating enzymes, and their use in the ubiquitinylation of E3 ligases and target substrate proteins. Ubiquitin antibody provided allows high sensitivity detection of ubiquitin and ubiquitin-conjugates.
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LYSO-ID® red cytotoxicity kit (GFP-CERTIFIED®) for microplates
Supplier: Enzo Life Sciences
A Rapid, Quantitative and HTS‐compatible Live Cell Cytotoxicity Assay
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ROS-ID™ superoxide detection kit
Supplier: Enzo Life Sciences
This kit is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry.
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Bacterial viability and gram stain kit
Supplier: Biotium
The Bacterial Viability and Gram Stain Kit contains three components: CF™488A-WGA, DAPI, and EthD-III solutions for distinguishing between gram-negative and gram-positive, as well as live versus dead bacteria.
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SOD activity kit
Supplier: Enzo Life Sciences
ELS offers a large collection of superoxide dismutase products for oxidative stress research.
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Ubiquitin conjugating kit (HeLa lysate-based)
Supplier: Enzo Life Sciences
1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway. 2. Ubiquitinylation of proteins of interest contained in exogenously added expression culture/cell extracts or tissue lysates/extracts, followed by their immediate detection/analysis or isolation/purification for use in subsequent experiments. 3. Modification of proteins using ubiquitin derivatives or mutants for improved detection/analysis or investigation of alternative (non-proteasomal) ubiquitin related pathways in subsequent experiments. For example, biotinylated-ubiquitin (Prod. No. BML-UW8705), for sensitive detection
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Hydrogen peroxide chemiluminescent detection Kit
Supplier: Enzo Life Sciences
For the quantitative determination of hydrogen peroxide in culture supernatants and cell lysates from any species.
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Calcium assay kits, FLIPR® Calcium 4 assay kit
Supplier: Molecular Devices
The FLIPR® Calcium 4 Assay Kit provides a fast, simple and reliable fluorescence-based assay for detecting changes in intracellular calcium.
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Red IHC chromogen (AP), HIGHDEF®
Supplier: Enzo Life Sciences
Red IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridisation systems.
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Green detection kit, LYSO-ID®
Supplier: Enzo Life Sciences
LYSO-ID® Green Detection Kit is an acidic organelle-selective dye specially designed to use with RFP-expressing cell lines, as well as cells expressing blue, cyan or orange fluorescent proteins (BFPs, CFPs, OFPs).
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