Supplier: SIGMA ALDRICH MICROSCOPY

This is a mounting medium (contains 45% acrylic resin and 55% xylenes) used in microscopy. It is an adhesive and specimen preservative that can be used manually and in automated coverslipping equipment. Special purpose adhesive and sealant for use with glass, non plastic and other non-reactive materials.

Supplier: Biotium

DNAzure® Blue Nucleic Acid Gel Stain is an ultrasensitive reagent for visible staining of dsDNA in agarose or polyacrylamide gels.

Supplier: SIGMA ALDRICH MICROSCOPY

To be used with SIAL 69571 Ren HY 956 (Hardener for Epoxy Embedding Medium)

Supplier: SIGMA ALDRICH MICROSCOPY

Hartman′s fixative, which is also known as Davidson′s Fixative, contains acetic acid, alcohol, and formalin. It is widely used for the preservation of eye, bone marrow, breast, testis, and other tissues for histologic examination

Supplier: SIGMA ALDRICH MICROSCOPY

Araldite® is an epoxy resin commonly used for embedding. It is basically free from polymerization damage and the plastic does not sublime in the microscope, hence providing continual support and preservation of fine detail.[1] It is composed of araldite M or CY 212, dodecenyl succinic anhydride, dibutyl phthalate and 2,4,6-tridimethylamine methyl phenol.

Supplier: Avantor

Formalin (dissolved formaldehyde) is used in histopathology laboratories.

Supplier: G-Biosciences

LabSafe GEL Blue™ is an enhanced protein stain based on Coomassie dye that offers unsurpassed sensitivity and rapid band visualisation.

Supplier: Biotium

Stains specifically designed for DNA in polyacrylamide gels.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: VWR Chemicals

Fixative, combining the coagulant qualities of alcohol, to enable a better fixation of nuclear and cytoplasmic elements. The erythrocytes are lysed and, therefore, do not obscure observation of the elements of interest. This fixative can be used according to the Saccomanno method.

Supplier: Thermo Fisher Scientific

Krypton fluorescent protein stain enables sensitive visualisation with fluorescence imagers (520 nm excitation; 580 nm emission) of proteins separated in polyacrylamide gels or transferred to membranes.

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion oil has been used in epifluorescent microscopy and cell identification.

Supplier: SIGMA ALDRICH MICROSCOPY

PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C.

Supplier: SIGMA ALDRICH MICROSCOPY

HistoChoice® is a patented, complex mixture of agents that allow tissues to retain their structure, antigenic sites and nucleic acid sites. This formulation in non-citrus, contains no mercury, formaldehyde or glutaraldehyde.

Supplier: Thermo Fisher Scientific

The Pierce reversible protein stain kit for nitrocellulose membranes is a rapid and sensitive alternative to ponceau S stain for protein detection on nitrocellulose membranes after transfer from polyacrylamide gels.

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce 6xhis protein tag stain reagent set is a fluorescent stain for preferential detection of histidine-tagged (his-tagged) recombinant fusion proteins in protein polyacrylamide gels.

Supplier: Thermo Fisher Scientific

Pierce silver stain for mass spectrometry is a complete kit for rapid and ultra-sensitive silver staining of proteins in polyacrylamide gels and efficient destaining of excised gel pieces for mass spectrometry analysis. This kit enables both first-time and experienced users to achieve consistent and reliable staining using high, low, and gradient-percentage gels in 1D and 2D formats. The optimised staining method ensures extremely sensitive staining while minimizsing covalent crosslinking of protein to the gel matrix, which can inhibit protein recovery. The destaining reagents facilitate complete removal of silver from stained protein bands and maximum protein recovery for subsequent mass spectrometry analysis.

Supplier: VWR Chemicals

Safemount Q Path® is a mounting medium, supplied in an PET bottle, which is compatible with Safesolv. It glues the coverglass to the slide and fixes the probe in position after staining.

Supplier: SIGMA ALDRICH MICROSCOPY

to be used with SIAL 78711 RenLam M-1 (Epoxy Embedding Medium)

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: SIGMA ALDRICH MICROSCOPY

CC/mount is an aqueous based permanent mounting medium with a very high refractive index, which when applied to the stained tissue sections can store the tissue specimens permanently without the fading of the chromogens. Because of the superior refractive index of CC/mount, tissues mounted in this medium look like specimens mounted with organic based mounting media. No coverslipping is required with CC/mount. However, if coverslipping is required, dried CC/mount can be post mounted by using an organic based mounting medium. CC/mount permits the long-term storage of antigens localised using organic insoluble chromogen substrates, including AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins. The high pH of CC/mount ensures increased stability of fluorescence intensity.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce Color Silver Stain Kit detects proteins electrophoresed in 1D and 2D polyacrylamide gels using metallic silver to produce color variations that facilitate differentiation of protein bands and spots.

Supplier: VWR Chemicals

When sectioning frozen tissue for rapid surgical biopsy diagnosis or for research (typically in Histochemistry), the tissue sample must be firmly attached to the microtome stage or chuck. Embedding material such as OCT are preferred as these also provide a supportive and protective aid to sectioning.

Supplier: Merck

Cryoembedding media for microscopy M-Freeze™ is used in the routine for preparing specimens of unfixed tissue (native material) of human origin for frozen-section analysis. It is also used for preparing cryo sections for enzyme and immunohistology methods to avoid fixation artefacts.

Supplier: VWR Collection

Immersion oil is used to increase microscope resolution.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: Thermo Fisher Scientific

GelCode blue safe protein stain is a fast and sensitive coomassie-dye reagent that provides one-step ultrasensitive detection of proteins in polyacrylamide gels and is formulated to avoid hazardous shipping.

Supplier: SIGMA ALDRICH MICROSCOPY

The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion Oil has a defined refractive index of about 1,516. It is important that the refractive index (nD) is about 1,5, similar to that of glass. This enables a homogeneous oil immersion to be achieved.

Supplier: Thermo Fisher Scientific

Pierce silver stain rescue reagent enables removal of undesirable background from unevenly and overly developed silver-stained polyacrylamide electrophoresis gels.