Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
ReadiLink™ DIG (Digoxigenin) Nick Translation dsDNA Labeling Kit
Supplier: AAT BIOQUEST
It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.
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ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit
Supplier: AAT BIOQUEST
It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.
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Buccutite™ Rapid Oligo Antibody Conjugation Kit
Supplier: AAT BIOQUEST
Optimised for labelling 100 µg protein. Buccutite™ rapid oligo antibody conjugation kit is optimised for conjugating 100 µg protein with an amino-modified oligo.
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ReadiLink™ Cy5 Oligo and ssDNA Labeling Kit
Supplier: AAT BIOQUEST
The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.
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Immobilised reductant column
Supplier: Thermo Fisher Scientific
Pierce™ Immobilised reductant column contains beaded resin to which an thiol-based reducing agent has been immobilised to enable solid-phase reduction of peptide and protein disulphide bonds.
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ReadiLink™ iFluor® 488 Oligo and ssDNA Labelling Kit
Supplier: AAT BIOQUEST
The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.
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Pierce™, EZ-Link™ Sulfhydryl Reactive Biotinylation Reagents
Supplier: Thermo Fisher Scientific
Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptides. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.
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ReadiLink™ iFluor® 647 Nick Translation dsDNA Labeling Kit
Supplier: AAT BIOQUEST
It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.
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ReadiLink™ xtra Rapid XFD555 Antibody Labelling Kit *BSA-Compatible, XFD555 Same Structure to Alexa Fluor™ 555* (2 Labellings)
Supplier: AAT BIOQUEST
XFD555 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 555 (Alexa Fluor® is the trademark of ThermoFisher).
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ReadiLink™ xtra Rapid XFD594 Antibody Labelling Kit *BSA-Compatible, XFD594 Same Structure to Alexa Fluor™ 594* (2 Labellings)
Supplier: AAT BIOQUEST
XFD594 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 594 (Alexa Fluor® is the trademark of ThermoFisher).
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ReadiLink™ xtra Rapid Cy3 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)
Supplier: AAT BIOQUEST
ReadiLink™ xtra rapid antibody labelling kits require essentially only 2 simple mixing steps without a column purification needed.
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ReadiLink™ Rapid XFD555 Antibody Labelling Kit *XFD555 Same Structure to Alexa Fluor™ 555* (2×50 µg Labellings)
Supplier: AAT BIOQUEST
XFD555 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 555 (Alexa Fluor® is the trademark of ThermoFisher).
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ReadiLink™ xtra Rapid XFD647 Antibody Labelling Kit *BSA-Compatible, XFD647 Same Structure to Alexa Fluor™ 647* (2 Labelings)
Supplier: AAT BIOQUEST
XFD647 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 647 (Alexa Fluor® is the trademark of ThermoFisher).
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ReadiLink™ Rapid iFluor® Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT BIOQUEST
Although FITC is still the most popular fluorescent labelling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence.
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ReadiLink™ xtra Rapid XFD750 Antibody Labelling Kit *BSA-Compatible, XFD750 Same Structure to Alexa Fluor™ 750* (2 Labellings)
Supplier: AAT BIOQUEST
XFD750 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 750 (Alexa Fluor® is the trademark of ThermoFisher).
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Buccutite™ Rapid PE-Texas Red Tandem Antibody Labelling Kit
Supplier: AAT BIOQUEST
Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE-Texas Red tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.
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Wheat Germ Agglutinin, XFD532 Labelled *XFD532 Same Structure to Alexa Fluor® 532*
Supplier: AAT BIOQUEST
XFD532, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 532, a trademark of ThermoFisher.
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Wheat Germ Agglutinin, XFD555 Labelled *XFD555 Same Structure to Alexa Fluor® 555*
Supplier: AAT BIOQUEST
XFD555, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 555, a trademark of ThermoFisher.
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Activated Horseradish Peroxidase (HRP), EZ-Link™ Plus
Supplier: Thermo Fisher Scientific
The Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase is an amine-reactive horseradish peroxidase (HRP) that provides higher conjugate yields (>95%) than glutaraldehyde chemistry.
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Cell Explorer™ Live Cell Tracking Kit
Supplier: AAT BIOQUEST
The kit uses a weakly fluorescent dye that carries a cell-retaining moiety.
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Buccutite™ Rapid Protein Crosslinking Kit *Microscale Optimized for Crosslinking 100 ug Antibody Per Reaction* (2 Labelings)
Supplier: AAT BIOQUEST
Protein-protein conjugations are commonly performed with a bifunctional linker such as SMCC.
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Fluorescence biotin quantitation kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Fluorescence Biotin Quantitation Kit requires only 10 μl of sample to accurately measure the biotinylation level of labeled antibodies and other biomolecules.
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ReadiLink™ Rapid mFluor™ Green 620 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT BIOQUEST
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.
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Azide reactive biotinylation reagents, EZ-Link™ Phosphine-PEG3-Biotin
Supplier: Thermo Fisher Scientific
EZ-Link® Phosphine-PEG3-Biotin is a versatile biotinylation reagent for labeling azide-containing molecules, which enables biotin-based detection and affinity purification of molecules via Staudinger ligation strategies.
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Controlled Protein-Protein Crosslinking Kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.
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ReadiLink™ Rapid iFluor® Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)
Supplier: AAT BIOQUEST
AAT Bioquest's iFluor® dyes are developed for labelling proteins, in particular, antibodies.
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Isobaric labelling reagent set, iodoTMTsixplex™
Supplier: Thermo Fisher Scientific
The iodoTMTsixplex™ labelling reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry.
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mFluor™ Violet 540-Wheat Germ Agglutinin (WGA) Conjugate
Supplier: AAT BIOQUEST
mFluor™ Violet 540 labelled WGA is well-excited by the violet laser, emitting a bright green fluorescence at 535 nm.
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mFluor™ Violet 500-Wheat Germ Agglutinin (WGA) Conjugate
Supplier: AAT BIOQUEST
mFluor™ Violet 500 labelled WGA is well-excited by the violet laser, emitting a bright green fluorescence at 501 nm.
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mFluor™ Violet 450-Wheat Germ Agglutinin (WGA) Conjugate
Supplier: AAT BIOQUEST
mFluor™ Violet 450 labelled WGA is well-excited by the violet laser, emitting a bright blue fluorescence at 445 nm.