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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

ReadiLink™ DIG (Digoxigenin) Nick Translation dsDNA Labeling Kit

ReadiLink™ DIG (Digoxigenin) Nick Translation dsDNA Labeling Kit

Supplier: AAT BIOQUEST

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

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ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit

ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit

Supplier: AAT BIOQUEST

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

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Buccutite™ Rapid Oligo Antibody Conjugation Kit

Supplier: AAT BIOQUEST

Optimised for labelling 100 µg protein. Buccutite™ rapid oligo antibody conjugation kit is optimised for conjugating 100 µg protein with an amino-modified oligo.

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ReadiLink™ Cy5 Oligo and ssDNA Labeling Kit

ReadiLink™ Cy5 Oligo and ssDNA Labeling Kit

Supplier: AAT BIOQUEST

The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.

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Immobilised reductant column

Immobilised reductant column

Supplier: Thermo Fisher Scientific

Pierce™ Immobilised reductant column contains beaded resin to which an thiol-based reducing agent has been immobilised to enable solid-phase reduction of peptide and protein disulphide bonds.

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ReadiLink™ iFluor® 488 Oligo and ssDNA Labelling Kit

ReadiLink™ iFluor® 488 Oligo and ssDNA Labelling Kit

Supplier: AAT BIOQUEST

The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.

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Pierce™, EZ-Link™ Sulfhydryl Reactive Biotinylation Reagents

Pierce™, EZ-Link™ Sulfhydryl Reactive Biotinylation Reagents

Supplier: Thermo Fisher Scientific

Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptides. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.

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ReadiLink™ iFluor® 647 Nick Translation dsDNA Labeling Kit

ReadiLink™ iFluor® 647 Nick Translation dsDNA Labeling Kit

Supplier: AAT BIOQUEST

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

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ReadiLink™ xtra Rapid XFD555 Antibody Labelling Kit *BSA-Compatible, XFD555 Same Structure to Alexa Fluor™ 555* (2 Labellings)

ReadiLink™ xtra Rapid XFD555 Antibody Labelling Kit *BSA-Compatible, XFD555 Same Structure to Alexa Fluor™ 555* (2 Labellings)

Supplier: AAT BIOQUEST

XFD555 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 555 (Alexa Fluor® is the trademark of ThermoFisher).

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ReadiLink™ xtra Rapid XFD594 Antibody Labelling Kit *BSA-Compatible, XFD594 Same Structure to Alexa Fluor™ 594* (2 Labellings)

ReadiLink™ xtra Rapid XFD594 Antibody Labelling Kit *BSA-Compatible, XFD594 Same Structure to Alexa Fluor™ 594* (2 Labellings)

Supplier: AAT BIOQUEST

XFD594 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 594 (Alexa Fluor® is the trademark of ThermoFisher).

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ReadiLink™ xtra Rapid Cy3 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)

ReadiLink™ xtra Rapid Cy3 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)

Supplier: AAT BIOQUEST

ReadiLink™ xtra rapid antibody labelling kits require essentially only 2 simple mixing steps without a column purification needed.

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ReadiLink™ Rapid XFD555 Antibody Labelling Kit *XFD555 Same Structure to Alexa Fluor™ 555* (2×50 µg Labellings)

ReadiLink™ Rapid XFD555 Antibody Labelling Kit *XFD555 Same Structure to Alexa Fluor™ 555* (2×50 µg Labellings)

Supplier: AAT BIOQUEST

XFD555 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 555 (Alexa Fluor® is the trademark of ThermoFisher).

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ReadiLink™ xtra Rapid XFD647 Antibody Labelling Kit *BSA-Compatible, XFD647 Same Structure to Alexa Fluor™ 647* (2 Labelings)

ReadiLink™ xtra Rapid XFD647 Antibody Labelling Kit *BSA-Compatible, XFD647 Same Structure to Alexa Fluor™ 647* (2 Labelings)

Supplier: AAT BIOQUEST

XFD647 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 647 (Alexa Fluor® is the trademark of ThermoFisher).

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ReadiLink™ Rapid iFluor® Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

ReadiLink™ Rapid iFluor® Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

Supplier: AAT BIOQUEST

Although FITC is still the most popular fluorescent labelling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence.

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ReadiLink™ xtra Rapid XFD750 Antibody Labelling Kit *BSA-Compatible, XFD750 Same Structure to Alexa Fluor™ 750* (2 Labellings)

ReadiLink™ xtra Rapid XFD750 Antibody Labelling Kit *BSA-Compatible, XFD750 Same Structure to Alexa Fluor™ 750* (2 Labellings)

Supplier: AAT BIOQUEST

XFD750 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 750 (Alexa Fluor® is the trademark of ThermoFisher).

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Buccutite™ Rapid PE-Texas Red Tandem Antibody Labelling Kit

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE-Texas Red tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.

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Wheat Germ Agglutinin, XFD532 Labelled *XFD532 Same Structure to Alexa Fluor® 532*

Wheat Germ Agglutinin, XFD532 Labelled *XFD532 Same Structure to Alexa Fluor® 532*

Supplier: AAT BIOQUEST

XFD532, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 532, a trademark of ThermoFisher.

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Wheat Germ Agglutinin, XFD555 Labelled *XFD555 Same Structure to Alexa Fluor® 555*

Wheat Germ Agglutinin, XFD555 Labelled *XFD555 Same Structure to Alexa Fluor® 555*

Supplier: AAT BIOQUEST

XFD555, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 555, a trademark of ThermoFisher.

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Activated Horseradish Peroxidase (HRP), EZ-Link™ Plus

Activated Horseradish Peroxidase (HRP), EZ-Link™ Plus

Supplier: Thermo Fisher Scientific

The Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase is an amine-reactive horseradish peroxidase (HRP) that provides higher conjugate yields (>95%) than glutaraldehyde chemistry.

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Cell Explorer™ Live Cell Tracking Kit

Cell Explorer™ Live Cell Tracking Kit

Supplier: AAT BIOQUEST

The kit uses a weakly fluorescent dye that carries a cell-retaining moiety.

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Buccutite™ Rapid Protein Crosslinking Kit *Microscale Optimized for Crosslinking 100 ug Antibody Per Reaction* (2 Labelings)

Buccutite™ Rapid Protein Crosslinking Kit *Microscale Optimized for Crosslinking 100 ug Antibody Per Reaction* (2 Labelings)

Supplier: AAT BIOQUEST

Protein-protein conjugations are commonly performed with a bifunctional linker such as SMCC.

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Fluorescence biotin quantitation kit, Pierce™

Fluorescence biotin quantitation kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Fluorescence Biotin Quantitation Kit requires only 10 μl of sample to accurately measure the biotinylation level of labeled antibodies and other biomolecules.

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ReadiLink™ Rapid mFluor™ Green 620 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

ReadiLink™ Rapid mFluor™ Green 620 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

Supplier: AAT BIOQUEST

AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.

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Azide reactive biotinylation reagents, EZ-Link™ Phosphine-PEG3-Biotin

Azide reactive biotinylation reagents, EZ-Link™ Phosphine-PEG3-Biotin

Supplier: Thermo Fisher Scientific

EZ-Link® Phosphine-PEG3-Biotin is a versatile biotinylation reagent for labeling azide-containing molecules, which enables biotin-based detection and affinity purification of molecules via Staudinger ligation strategies.

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Controlled Protein-Protein Crosslinking Kit, Pierce™

Controlled Protein-Protein Crosslinking Kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.

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ReadiLink™ Rapid iFluor® Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

ReadiLink™ Rapid iFluor® Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

Supplier: AAT BIOQUEST

AAT Bioquest's iFluor® dyes are developed for labelling proteins, in particular, antibodies.

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Isobaric labelling reagent set, iodoTMTsixplex™

Isobaric labelling reagent set, iodoTMTsixplex™

Supplier: Thermo Fisher Scientific

The iodoTMTsixplex™ labelling reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry.

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mFluor™ Violet 540-Wheat Germ Agglutinin (WGA) Conjugate

mFluor™ Violet 540-Wheat Germ Agglutinin (WGA) Conjugate

Supplier: AAT BIOQUEST

mFluor™ Violet 540 labelled WGA is well-excited by the violet laser, emitting a bright green fluorescence at 535 nm.

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mFluor™ Violet 500-Wheat Germ Agglutinin (WGA) Conjugate

mFluor™ Violet 500-Wheat Germ Agglutinin (WGA) Conjugate

Supplier: AAT BIOQUEST

mFluor™ Violet 500 labelled WGA is well-excited by the violet laser, emitting a bright green fluorescence at 501 nm.

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mFluor™ Violet 450-Wheat Germ Agglutinin (WGA) Conjugate

mFluor™ Violet 450-Wheat Germ Agglutinin (WGA) Conjugate

Supplier: AAT BIOQUEST

mFluor™ Violet 450 labelled WGA is well-excited by the violet laser, emitting a bright blue fluorescence at 445 nm.

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