Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Phosphate, 0,2 M buffer solution, pH 4,4

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,2 M, pH value: 4,4, Liquid

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IEF Cathode buffer (10X) with lysine and arginine

Supplier: Thermo Fisher Scientific

Running Buffer, IEF Cathode buffer (10X) with lysine and arginine

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pH buffer solutions, referenced at 20 °C, traceable to SRM from NIST, Fluka™

Supplier: Honeywell Chemicals

pH Buffer solution, Phosphate, pH value: 7,00, ±0.02, Reference temperature: 20 °C, Fluka™, 6×1 L

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Triethylammonium acetate buffer, BioUltra, volatile buffer, Fluka®

Supplier: Honeywell Chemicals

Triethylammonium acetate buffer, 1,0 M in H₂O, pH value: 7,0

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Laemmli SDS sample buffer, non-reducing (4X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, non-reducing, 4X

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GST buffer kit

Supplier: Cytiva

GST Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of GST-tagged proteins according to recommended protocols.

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Buffer Capsules

Supplier: Greisinger

Cost-effective alternative: Buffer capsules and working sets. With buffer capsules, you can produce a 100 ml buffer solution in the most cost-effective manner. A particular advantage of the capsules is that their storage life is several years longer than that of ready-to-use solutions.

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Tris-Glycine (TG) buffer,, MP Biomedicals

Supplier: MP Biomedicals

Tris-glycine buffer is used for western blotting and gel electrophoresis.

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10X Phosphate Buffered Saline (PBS), Sterile, Dulbecco's Formula, without Magnesium and Calcium

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)

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Buffer solutions

Supplier: SI Analytics

Buffer solutions intended for extremely precise calibration.

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His buffer kit

Supplier: Cytiva

His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, a method for optimization of imidazole concentration in the binding buffer is described.

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Protein A/G HP SpinTrap™ Buffer Kit

Supplier: Cytiva

Protein A/G HP SpinTrap™ Buffer kit is optimised for standard affinity-based enrichment/immunoprecipitation according to the protocol provided with protein A HP SpinTrap™, protein G HP SpinTrap™, and Ab SpinTrap™ columns.

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Ab buffer kit

Supplier: Cytiva

Ab buffer kit contains prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols.

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IPG buffers

Supplier: Cytiva

IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.