Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Ionisation buffer solutions (CRMs), for AAS and ICP, Fluka™

Supplier: Honeywell Chemicals

Matrix Modifier, 2% Lithium (Li) in 1% HNO₃

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Buffers, imidazole, 0,5 M solutions

Supplier: Thermo Fisher Scientific

Imidazole buffer solution, 0,5 M, pH value: 7,0, Liquid

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Glycine, 0.2M buffer soln., pH 3.5

Supplier: Thermo Fisher Scientific

Glycine buffer solution, 0,2 M, pH value: 3,5, Odourless, Liquid

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Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula

Supplier: Thermo Fisher Scientific

Cell culture buffer, Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula

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IEF Cathode buffer (10X) with lysine

Supplier: Thermo Fisher Scientific

Running Buffer, IEF Cathode buffer (10X) with lysine

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Laemmli SDS sample buffer, reducing (4X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, reducing, 4X

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Buffers, MOPS, 1,0 M solutions

Supplier: Thermo Fisher Scientific

MOPS buffer solution pH 6,5 (1 mol/l), pH value: 6,5, Colourless, Odourless, Liquid

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Buffers, concentrated solutions, Fluka™

Supplier: Honeywell Chemicals

pH Buffer, concentrate, pH value: 4,00, ±0.02, Ampoule

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Enzyme storage buffer in PBS and glycerol, autoclaved

Supplier: Thermo Fisher Scientific

Enzyme Storage Buffer in PBS and Glycerol

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LDS-sample buffer (4X), non-reducing

Supplier: Thermo Fisher Scientific

LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.

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Phosphate-buffered saline (PBS, 10X), no potassium

Supplier: Thermo Fisher Scientific

Phosphate-buffered saline (PBS), No potassium, 10X, Liquid

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Tris-HCl, 1M solution, pH 8,0, Molecular Biology Grade, Ultrapure

Supplier: Thermo Fisher Scientific

1M solution of Tris, Ultrapure in high purity distilled H₂O, adjusted to pH 8,0.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: LONZA

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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Buffer, TRIS, 1,0 M Solution, pH 9,0

Supplier: Thermo Fisher Scientific

TRIS buffer solution pH 9 (1 mol/L), Odourless, Liquid

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10% SDS (Sodium Dodecyl Sulfate), Corning®

Supplier: Corning

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.