Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®
Supplier: Corning
20X Saline-Sodium Citrate (SSC) buffer is used in both nucleic acid hybridization and blot transfer procedures
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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer), Millipore®
Supplier: Merck Millipore (Calbiochem)
Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer)
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Tris Hydrochloride
Supplier: Merck Millipore (Calbiochem)
Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells.: This study highlights the application of TRIS Hydrochloride in blocking TRPM7 channels, which is significant for cell signaling and ion channel regulation in pharmacological research (Holderby & Kozak, 2024).
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Separating or resolving buffer (4X)
Supplier: Thermo Fisher Scientific
Separating Buffer, Separating or Resolving Buffer, 4X
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Tris-HCl, 1M solution, pH 8,0, Molecular Biology Grade, Ultrapure
Supplier: Thermo Fisher Scientific
1M solution of Tris, Ultrapure in high purity distilled H₂O, adjusted to pH 8,0.
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Maleate buffers, 0,2 M solutions, pH 5,0 - 7,0
Supplier: Thermo Fisher Scientific
Maleate buffer, 0,2 M, pH value: 6,5, Liquid
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Buffers, MES, 0,5 M solutions
Supplier: Thermo Fisher Scientific
MES, buffer solution, 0,5 M, pH value: 5,0, Colourless, Liquid
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Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula
Supplier: Thermo Fisher Scientific
Cell culture buffer, Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula
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Buffers, MOPS, 1,0 M solutions
Supplier: Thermo Fisher Scientific
MOPS buffer solution pH 7,5 (1 mol/l), pH value: 7,5, Colourless, Odourless, Liquid
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Buffers, imidazole, 0,5 M solutions
Supplier: Thermo Fisher Scientific
Imidazole buffer solution, 0,5 M, pH value: 6,5, Liquid
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TE buffer, 1X, ready to use, pH 8,0, low EDTA, Molecular Biology Grade, Affynmetrix/USB
Supplier: Thermo Fisher Scientific
TE buffer with low EDTA. Use directly to re-suspend and/or dilute purified DNA or RNA.
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Glycine, 0.2M buffer soln., pH 3.5
Supplier: Thermo Fisher Scientific
Glycine buffer solution, 0,2 M, pH value: 3,5, Odourless, Liquid
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IEF Cathode buffer (10X) with lysine
Supplier: Thermo Fisher Scientific
Running Buffer, IEF Cathode buffer (10X) with lysine
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Buffers, BIS-TRIS propane, 0,2 M solutions
Supplier: Thermo Fisher Scientific
2,2'-(Propane-1,3-diyldiimino)bis[2-(hydroxy methyl)propane-1,3-diol]
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Ionisation buffer solutions (CRMs), for AAS and ICP, Fluka™
Supplier: Honeywell Chemicals
Matrix Modifier, 2% Lithium (Li) in 1% HNO₃
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Buffer, TRIS, 1,0 M Solution, pH 9,0
Supplier: Thermo Fisher Scientific
TRIS buffer solution pH 9 (1 mol/L), Odourless, Liquid
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