You searched for: Assays
An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.
Calcineurin cellular activity assay kit
Supplier: ENZO LIFE SCIENCES
A complete colorimetric assay kit for measuring cellular calcineurin (PP2B) phosphatase activity. It employs a convenient 96-well plate format with all reagents necessary for measuring calcineurin (PP2B) phosphatase activity in tissue/cellular extracts PLUS human recombinant calcineurin is included as a positive control.
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PPARδ transcription factor assay kit
Supplier: Cayman Chemical
A 96-well assay for measurement of PPARδ DNA binding activity.
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Cell proliferation assay kit, WST-1
Supplier: Cayman Chemical
WST-1 Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. The assay is based on the reduction of tetrazolium salt WST-1 to soluble formazan by electron transport across the plasma membrane of dividing cells. This kit will also allow investigators to screen drug candidates involved in regulation of cell cycle.
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FLUOR DE LYS® HDAC fluorometric cellular activity assay kit
Supplier: ENZO LIFE SCIENCES
The HDAC fluorometric cellular activity assay allows the determination of deacetylase activity within an undisturbed cellular environment and provides accurate activity information that is reflective of endogenous regulation. It also allows the study of the effects of upstream regulators on deacetylase activity and the detection of inhibitors or activators that act indirectly to affect deacetylase activity.
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Fluorescent protease assay, Fluoro™
Supplier: G-Biosciences
The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labelled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the proteases present in the sample of interest digest the FITC-casein substrate into smaller peptides, the quenching of the fluorescence label is relieved and the fluorescence of the substrate is increased. The fluorescence of the FITC-labeled peptide is measured with excitation at 485 nm and emission at 530 nm to determine protease activity. The kit detects picogram level of proteases present in the sample.
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LEADING LIGHT® Wnt cell line assay reagents
Supplier: ENZO LIFE SCIENCES
For use with the LEADING LIGHT® Wnt Reporter Cell Line.
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Reagents for Color-de-Lys™ HDAC colorimetric activity assay kit
Supplier: ENZO LIFE SCIENCES
Cellular assay, Color-de-Lys™ deacetylated standard, 30 µl
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Protease assay kit
Supplier: G-Biosciences
This Protease Assay Kit is designed for the quantitative determination of proteases present in protein sample, using a dye-labelled protein substrate. The proteases present in the sample of interest will digest the protein substrate and release dye labelled peptides. The absorbance of the dye-labelled peptide is measured at 570 nm for determination of protease activity.
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Creatinine (urinary) colorimetric assay kit
Supplier: Cayman Chemical
For the measurement of creatinine levels in urine.
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Oct4 transcription factor assay kit
Supplier: Cayman Chemical
96-Well assay for measurement of Oct4.
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Reagents for Fluor-de-Lys®-Green HDAC fluorometric activity assay kit
Supplier: ENZO LIFE SCIENCES
Cellular assay, Fluor-de-Lys®-green substrate, in 50 mM solution in DMSO
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Cellular senescence live cell analysis assay
Supplier: ENZO LIFE SCIENCES
Simple assay to quantify senescence-associated β-galactosidase activity, in live cells, using flow cytometry or fluorescent microscopy.
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NETosis Assay Kit
Supplier: Cayman Chemical
For the measurement of NET-derived neutrophil elastase.
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GFP-CERTIFIED® FLUOFORTE® Calcium assay kit
Supplier: ENZO LIFE SCIENCES
The GFP-Certified™ FluoForte™ Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.
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PROTEOSTAT® thermal shift stability assay kit
Supplier: ENZO LIFE SCIENCES
This kit has been designed for monitoring protein stability under systematic thermal stress conditions.
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VWR® Fluorescent DNA Quantitation Kit
Supplier: VWR Chemicals
Fluorescent DNA Quantitation Kit uses fluorescent bisBenzimide H33258 dye to bind to AT sequences of the minor groove of double-stranded DNA. In the assay, the sample is excited at 360 nm and when the dye binds to dsDNA an emission spectrum at 460 nm is produced.
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Calcium assay kit
Supplier: Cayman Chemical
For the determination of total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates.
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Cell viability assay kits, EarlyTox™ Caspase-3/7 R110 assay kit
Supplier: Molecular Devices
EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.
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Calcium assay kits, FLIPR® Calcium 4 assay kit
Supplier: Molecular Devices
The FLIPR® Calcium 4 Assay Kit provides a fast, simple and reliable fluorescence-based assay for detecting changes in intracellular calcium.
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Potassium assay kits, FLIPR®
Supplier: Molecular Devices
The FLIPR® Potassium Assay Kit measures functional activity of ligand- and voltage-gated potassium channels. The homogeneous, no-wash assay protocol provides a large signal window and high Z' values.
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Steady-Luc™ Firefly HTS assay kit (lyophilised)
Supplier: Biotium
Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.
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Membrane potential assay kit, FLIPR®
Supplier: Molecular Devices
The FLIPR® Membrane Potential Assay Kit provide a fast, simple and reliable, fluorescence-based assay for detecting changes in voltage across the cell membrane and its homogenous fluorescence-based formulations for observation of real-time membrane potential changes associated with ion channel activation and ion transporter proteins.
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ViaFluor® 488 live cell microtubule stain 1000X in DMSO
Supplier: Biotium
Stain microtubules in live cells with green fluorescence.
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Caspase-3 fluorometric assay kit
Supplier: ENZO LIFE SCIENCES
Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate DEVD-AFC.
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Cell counting kit-F
Supplier: ENZO LIFE SCIENCES
Fluorometric kit for simple and accurate cell proliferation and cytotoxicity assays. Advantages includes no radioisotopes, more sensitive than the colorimetric assay, short staining reaction time, no solubilization step, correlates with the [3H]-thymidine incorporation assay.
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ROS Detection Cell-Based Assay Kit (DHE)
Supplier: Cayman Chemical
Measure superoxide and hydrogen peroxide levels in living cells.
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Caspase-2 fluorometric assay kit
Supplier: ENZO LIFE SCIENCES
Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate VDVAD-AFC.
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ApoSENSOR™ ADP/ATP ratio assay kit
Supplier: ENZO LIFE SCIENCES
The assay is designed to detect ADP/ATP ratio. The changes in ADP/ATP ratios have been used to conveniently differentiate apoptosis from necrosis.
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CREB (Phospho-Ser133) transcription factor assay kit
Supplier: Cayman Chemical
A 96-well assay for measurement of CREB.