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Assays

An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.

Glucose (HK) assay kit

Supplier: Merck

The Glucose (HK) Assay Kit is for the quantitative, enzymatic determination of glucose in food and other material.

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VWR®, Bradford protein assay, proteomics grade

VWR®, Bradford protein assay, proteomics grade

Supplier: VWR Chemicals

The Bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. Based upon complex formation between basic and aromatic amino acid residues with Coomassie® Brilliant Blue G-250 dye, the Bradford method is easier, faster, and more sensitive than the Lowry method.

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Bicinchoninic acid disodium salt hydrate

Supplier: SIGMA ALDRICH MICROSCOPY

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+) in the presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

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VWR® Protein Precipitation Kit

VWR® Protein Precipitation Kit

Supplier: VWR Chemicals

The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.

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SREBP-2 transcription factor assay kit

SREBP-2 transcription factor assay kit

Supplier: Cayman Chemical

96 well assay for measurement of SREBP-2.

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Triglyceride colorimetric assay kit

Supplier: Cayman Chemical

Triglyceride Colorimetric Assay Kit is a simple, reproducible and sensitive tool for assaying triglycerides in plasma, serum, cell lysates and tissue homogenate samples.

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Cholesterol cell-based detection assay kit

Supplier: Cayman Chemical

Cholesterol Cell-Based Detection Assay Kit is a 192-well assay for measurement of cholesterol.

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Uric acid assay kit

Uric acid assay kit

Supplier: Cayman Chemical

Uric Acid Assay Kit is used to measure uric acid in serum, plasma and urine.

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Phagocytosis assay kit (IgG FITC)

Supplier: Cayman Chemical

Phagocytosis Assay Kit (IgG FITC) for the identification of factors regulating the phagocytic process in vitro.

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PPARα transcription factor assay kit

PPARα transcription factor assay kit

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARα DNA binding activity.

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SREBP-1 transcription factor assay kit

SREBP-1 transcription factor assay kit

Supplier: Cayman Chemical

A 96-well assay for measurement of SREBP-1.

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APO-BRDU™ kit, TUNEL Assay

Supplier: ENZO LIFE SCIENCES

Cellular assay, APO-BRDU™ kit

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Firefly luciferase assay kit (Lyophilised)

Supplier: Biotium

This kit is designed to measure firefly luciferase activity in transfected cell reporter assays.

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Red hydrogen peroxide assay kit

Supplier: ENZO LIFE SCIENCES

Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is suitable for both 96- and 348-well formats and is compatible with HTS liquid handling.

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ATP-Glo™ Bioluminometric cell viability assay

ATP-Glo™ Bioluminometric cell viability assay

Supplier: Biotium

ATP-Glo™ Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay involves a single addition of ATP-Glo™ Detection Cocktail directly to cells in culture medium.

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Protein assay kits, RED 660™

Protein assay kits, RED 660™

Supplier: G-Biosciences

RED 660™ Protein Assay is a single reagent colorimetric assay that outperforms many commercial colorimetric assays, including Bradford and improved Coomassie/Bradford assays. It offers greater linearity, colour stability, and compatibility with detergents, reducing agents and other interfering agents compared to the Coomassie assays. The single, ready to use reagent allows for rapid analysis of total protein concentration and generates highly reproducible results.

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Reporter gene assays, SpectraMax® DuoLuc™ reporter assay kit

Reporter gene assays, SpectraMax® DuoLuc™ reporter assay kit

Supplier: Molecular Devices

SpectraMax® DuoLuc™ reporter assay kit offers sensitive detection of dual luciferase reporter gene assays, which are important tools for monitoring gene expression and other cellular events.

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Protein carbonyl colorimetric assay kit

Supplier: Cayman Chemical

The Protein Carbonyl Colorimetric Assay Kit is for the measurement of oxidised proteins. Protein samples are derivatised by making use of the reaction between 2,4-dinitrophenylhydrazine and protein carbonyls.

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NF-κB (p65) transcription factor assay kit

NF-κB (p65) transcription factor assay kit

Supplier: Cayman Chemical

NF-κB (p65) Transcription Factor Assay Kit is a 96-well assay for measurement of NF-κB (p65).

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PROTEOSTAT® protein aggregation assay

PROTEOSTAT® protein aggregation assay

Supplier: ENZO LIFE SCIENCES

PROTEOSTAT® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecular chaperone activity. The assay can be employed to streamline protein processing and formulation optimization procedures.

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Protein quantitation assay kits, Non-Interfering™ (NI™) Protein Assay

Protein quantitation assay kits, Non-Interfering™ (NI™) Protein Assay

Supplier: G-Biosciences

A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt, and others.

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Mouse anti-type II collagen IgG assay kit (bovine)

Mouse anti-type II collagen IgG assay kit (bovine)

Supplier: Cayman Chemical

An immunometric assay for the measurement of type II collagen antibody in plasma and serum.

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ApoSENSOR™ Cell viability assay kit

Supplier: ENZO LIFE SCIENCES

The assay utilizes bioluminescent detection of the ATP level for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin. The light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).

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p53 transcription factor assay kit

p53 transcription factor assay kit

Supplier: Cayman Chemical

96-Well assay for measurement of p53.

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Cytochrome C releasing apoptosis assay kit

Supplier: ENZO LIFE SCIENCES

For the detection of Cytochrome c translocation from mitochondria into cytosol during apoptosis.

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FLUOR DE LYS®-Green HDAC2 fluorometric drug discovery assay kit

Supplier: ENZO LIFE SCIENCES

The HDAC2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or kinetic assay of the enzyme under varying conditions.

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VWR® Fluorescent DNA Quantitation Kit

VWR® Fluorescent DNA Quantitation Kit

Supplier: VWR Chemicals

Fluorescent DNA Quantitation Kit uses fluorescent bisBenzimide H33258 dye to bind to AT sequences of the minor groove of double-stranded DNA. In the assay, the sample is excited at 360 nm and when the dye binds to dsDNA an emission spectrum at 460 nm is produced.

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Calcium assay kit

Supplier: Cayman Chemical

For the determination of total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates.

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Calcium assay kits, FLIPR® Calcium 4 assay kit

Calcium assay kits, FLIPR® Calcium 4 assay kit

Supplier: Molecular Devices

The FLIPR® Calcium 4 Assay Kit provides a fast, simple and reliable fluorescence-based assay for detecting changes in intracellular calcium.

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Protein quantitation assay kits, CB-X™ Protein assay

Protein quantitation assay kits, CB-X™ Protein assay

Supplier: G-Biosciences

CB-X™ Protein Assay is a Coomassie dye based protein assay based on the Bradford Protein Assay. For protein samples in simple, uncomplicated aqueous buffers CB-X™ is a highly sensitive, single reagent assay that can be performed in 5 minutes. For complicated protein samples, CB-X™ Protein Assay is supplied with reagents to clean up the samples and remove all reagents and chemicals that interfere with accurate protein estimation in a single step. These reagents include detergents, chaotropes, reducing agents, alkylating agents, sugars, high salt concentrations, buffering agents and chelating agents. The clean up stage and subsequent protein assay is performed in a single tube to ensure no protein loss and to maintain the accuracy of the assay.

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