You searched for: Nucleic Acid Purification Kits and Reagents
Plasmid isolation, single-strand phage DNA, M-13 isolation kits, E.Z.N.A.® and E-Z 96®
Supplier: OMEGA BIO-TEK
E.Z.N.A.® M13 DNA kits are designed to purify up to 10 μg of single-stranded DNA from up to 3 ml of phage supernatant. Yields of single-stranded DNA obtained using E.Z.N.A.® M13 DNA kits are around 3 to 10 μg and reproducible when the isolations are performed from the same culture. Kits are also available in E-Z 96® DNA plate format.
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E.Z.N.A.® Plant DNA DS kit
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® Plant DNA DS Mini Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols or having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridisation applications.
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E.Z.N.A.® SP plant DNA kit
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® SP plant DNA kit is specially designed for the rapid and reliable isolation of high quality total cellular DNA from a variety of plant species. Up to 100 mg of fresh samples or 30 mg of dry samples can be processed in less than 40 minutes. Purified DNA is suitable for most downstream applications including PCR, restriction digestions and hybridisation techniques.
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Mag PCR normalizer kit, Axygen® AxyPrep
Supplier: Corning
The AxyPrep™ Mag PCR Normalizer Kit utilises a paramagnetic bead-based purification system for PCR clean up.
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NUCLEIC-CARD™ Nucleic Acid Stabilisation and Archiving Devices
Supplier: Copan
NUCLEIC-CARD™ is specifically designed to collect, transport, and store human DNA from buccal cells, saliva, blood, etc. Thanks to the unique chemical treatment, NUCLEIC-CARD™ preserves DNA for 20 years at room temperature.
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Fungal DNA kits, E.Z.N.A.®
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® Fungal DNA kits allow for the rapid and reliable isolation of high quality total cellular DNA from a wide variety of fungal species without the need for organic extraction.
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DNA mini columns for genomic/plasmid DNA extraction, Hi-Bind®
Supplier: OMEGA BIO-TEK
Hi-Bind® DNA mini columns are silica glass fibre columns that can bind up to 100 μg of genomic DNA or 35 μg of plasmid DNA per prep. Columns feature an attached lid and a vacuum luer for processing with vacuum manifolds and by centrifugation.
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AxyPrep™ FragmentSelect-I kit, Axygen®
Supplier: Corning
The AxyPrep™ FragmentSelect-I Kit is optimized for Illumina next generation sequencing platforms and Life Technologies (SOLiD) fragment size selection needs to simplify the library construction workflow. The kit is designed for fragment size selection needs in the Roche/454’s DNA Genome sequencer library construction workflow.
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Mag-Bind® stool DNA 96 kit
Supplier: OMEGA BIO-TEK
The Mag-Bind® Stool DNA 96 Kit allows rapid and reliable isolation of high-quality genomic DNA (both pathogen and host DNA) from stool samples.
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Bood DNA HV kit, CE IVD, Mag-Bind®
Supplier: OMEGA BIO-TEK
The Mag-Bind® Blood DNA HV Kit CE IVD is designed for rapid and reliable isolation of high-quality genomic DNA from up to 4 ml whole blood samples.
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Resin, StrataClean
Supplier: AGILENT
StrataClean resin eliminates the need to perform phenol:chloroform extractions, which are highly toxic and combustible.
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Universal pathogen DNA kit, Mag-Bind®
Supplier: OMEGA BIO-TEK
The Mag-Bind® Universal Pathogen DNA 96 Kit allows rapid and reliable isolation of high-quality pathogen and host genomic DNA from tissue samples. Up to a 30 mg tissue sample is recommended and can be processed in less than 60 minutes. The system allows for automation after sample lysis via Hamilton STAR™, Thermo KingFisher™ Flex, Applied Biosystems® MagMAX™, Qiagen Biosprint, and other liquid handling instruments.
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E.Z.N.A.® Blood DNA Kit
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® blood DNA kit provides rapid total DNA isolation from up to 250 µl of fresh or frozen anticoagulated whole blood. The E.Z.N.A.® blood DNA kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, bone marrow, lymphocytes, platelets, and body fluids. This kit allows for simultaneous processing of single or multiple samples in less than 30 minutes. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol or ethanol have been eliminated. DNA purified with the E.Z.N.A.® blood DNA method is ready for applications such as PCR, southern blotting, or restriction enzyme digestion.
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E.Z.N.A.® Plasmid DNA Mini Kit I, (V-spin)
Supplier: OMEGA BIO-TEK
Isolate up to 25 µg plasmid DNA from 1 to 5 ml bacterial cultures using mini spin columns.
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E.Z.N.A.® Plasmid DNA Mini Kit I, (Q-spin, capless)
Supplier: OMEGA BIO-TEK
Isolate up to 25 µg plasmid DNA from 1 to 5 ml bacterial cultures using mini spin columns.
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EX-WAX™ Paraffin-Embedded DNA Extraction Kit
Supplier: Merck
CHEMICON's EX-WAX™ DNA Extraction Kit is intended to extract DNA from paraffin-embedded tissue fixed in 10% Formalin or non-crosslinking fixatives. DNA extracted by this kit is suitable for amplification by PCR.
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E.Z.N.A.® Tissue DNA Kit, E.Z.N.A®
Supplier: OMEGA BIO-TEK
Isolate DNA from tissues, buccal swabs, cultured cells, whole blood, body fluids, paraffin-embedded tissues, and mouse tail snips using mini spin columns.
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E.Z.N.A.® Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue DNA Kits
Supplier: OMEGA BIO-TEK
Selected protease digestion releases microgram amounts of DNA from FFPE samples.
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UDI adapters (1-96), sparQ
Supplier: Quantabio
sparQ UDI Adapters (1-96) are unique dual-indexed adapters designed to be used on illumina sequencing platforms. It is compatible with both DNA and RNA libraries. The use of UDI’s minimises index hopping events while also enabling greater multiplexing on flow cells.
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MicroElute RNA clean up kit, E.Z.N.A.®
Supplier: OMEGA BIO-TEK
The MicroElute RNA clean-up kit is designed to purify and concentrate RNA (from 50 μg down to picogram amounts) from enzymatic reactions or for desalting RNA samples (e.g. following phenol extraction and ethanol precipitation). The procedure uses lower elution volumes, making it well suited for applications require highly concentrated RNA sample.
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Mag-Bind® DNA/RNA Kit
Supplier: OMEGA BIO-TEK
High-throughput DNA and RNA isolation from cultured cells and tissue using magnetic beads.
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E-Z Select® 24-Well Plate, 25 ml
Supplier: OMEGA BIO-TEK
E-Z Select® 24-well Plate, 25 ml is designed for performing large volume applications on automated platforms such as Hamilton Microlab® STAR or equivalent.
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Tissue DNA kit, genomic DNA isolation from tissue, E-Z 96®
Supplier: OMEGA BIO-TEK
By adapting HiBind® technology with 96-well plates, the E-Z 96® Tissue DNA kit provides a high throughput method to purify genomic DNA from whole blood, buccal swabs, mouse tail, rat tail, tissues and animal cells and tissues in a 96-well plate format. Purified DNA is suitable for most downstream applications such as PCR, restriction enzyme digestion, and hybridisation techniques.
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Genomic DNA isolation from blood, E.Z.N.A.® SQ blood DNA kit
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® SQ blood DNA kit provides a reliable method for the isolation of high molecular weight genomic DNA from fresh, frozen, or anti-coagulated whole blood. The method can also be used for preparation of genomic DNA from buffy coat, bone marrow or cultured cells. There is no need for phenol/chloroform extractions, and time consuming steps such as CsCl gradient ultracentrifugation are eliminated. DNA purified using the E.Z.N.A.® SQ blood DNA method is ready for downstream applications such as PCR, southern blotting and restriction enzyme digestion.
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E.Z.N.A.® Universal Pathogen DNA Kit
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® Universal Pathogen Kit allows for the rapid and reliable isolation of high quality host genomic DNA, gram positive and negative bacterial DNA, fungal spore DNA and viral DNA and viral RNA from tissue, urine, serum and fecal samples.
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Genomic DNA isolation
Supplier: AGILENT
The RecoverEase DNA isolation kit recovers high-molecular-weight (100 to 500 kb) genomic DNA that is fully hydrated and ready for multiple applications the next day. DNA can be isolated from whole tissues such as liver, spleen, kidney, brain and testes without hazardous and tedious organic extractions or ethanol precipitations. The RecoverEase DNA isolation kit quickly isolates high-molecular-weight genomic DNA from a variety of tissues without organic solvent extractions or ethanol precipitation.
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RNA isolation, total RNA kit, E.Z.N.A.® MicroElute
Supplier: OMEGA BIO-TEK
E.Z.N.A.® MicroElute Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amounts of cultured eukaryotic cells, tissues such as as laser dissected samples (LDS) or fine needle aspirates(FNA). Normally, up to 5×10⁵ eukaryotic cells or 5 mg tissue can be used in a single experiment depending on the type of tissue used. This kit allows processing of single or multiple of samples in less than 30 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifuation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10 to 15 µl nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern blotting, Poly A+ purification, nuclease protection and invitro translation.
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PCR clean-up kit, Axygen® AxyPrep™ Mag
Supplier: Corning
The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilises a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimised buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.
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Tissue DNA kit, genomic DNA isolation from tissue, E.Z.N.A.®
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® tissue DNA kit offers a simple, rapid, and cost effective method for the isolation of DNA from a wide variety of sample sources including fresh or frozen animal cells and tissues. After cell lysis, the DNA purification process can be completed in less than 30 minutes. Up to 30 mg of tissue at a time can be readily processed at a time using the simple E.Z.N.A.® tissue DNA protocol. Single or multiple samples can be simultaneously processed with this spin-column based kit. There is no need for phenol/chloroform extractions, or time-consuming steps such as precipitation with isopropanol or ethanol. DNA purified using the E.Z.N.A.® tissue DNA kit is ready for most downstream applications such as PCR, southern blot and restriction enzyme digestion.
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Viral DNA/RNA Xpress kit, CE IVD, Mag-Bind®
Supplier: OMEGA BIO-TEK
Mag-Bind® Viral DNA/RNA Xpress Kit CE IVD follows a magnetic bead-based approach for the rapid and reliable isolation of viral DNA and RNA from nasopharyngeal (NP) swab specimens that are dry or in viral transport media (VTM), from saliva and other sample sources.