You searched for: qPCR/RT-qPCR Enzymes and Kits
qScript™ One-Step qRT-PCR kits
Supplier: Quantabio
Synthesis of cDNA and PCR amplification are carried out in the same tube without opening between procedures. These kits are optimised to deliver maximum RT-PCR efficiency and specificity, enabling unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes. As a result the qScript™ One-Step qRT-PCR Kits offer improved sensitivity over other commercially available qPCR reagents. The qScript™ One-Step Master Mix maximises the activities of both reverse transcriptase and PCR enzymes, while minimising primer-dimer formation. High specificity is assured with AccuStart™ Taq DNA Polymerase, which is bound by monoclonal antibodies for superior hot-start. Upon heating to 95 °C, the antibodies denature irreversibly releasing fully active and unmodified Taq DNA polymerase. qScript™ One-Step qRT-PCR Kits are available for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye.
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Verso™ qRT-PCR Kits
Supplier: Thermo Fisher Scientific
The Verso™ qRT-PCR system combines the Verso™ RT enzyme with ABsolute™ Blue qPCR master mix to provide maximum convenience and versatility. Available in a 1- or 2-step format, as well as multiple pack sizes, to suit all application and throughput requirements.
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RT-PCR kits, Verso™
Supplier: Thermo Fisher Scientific
The Verso™ RT-PCR system combines a reverse transcription enzyme, improved priming options and optimised buffers to generate high yield and full length cDNA. With a variety of kit options for both 1- and 2-step RT-PCR reactions, Verso™ provides optimum flexibility and sensitivity in an easy to use format.
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VWR® One-Step RT-qPCR Kit
Supplier: VWR Chemicals
VWR® One-Step RT-qPCR Kit is designed to facilitate rapid, sensitive, and precise detection and quantification of various RNA sequences via dye-based qPCR. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.
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VWR® Probe One-Step RT-qPCR Kit
Supplier: VWR Chemicals
VWR® Probe One-Step RT-qPCR Kit is designed to facilitate rapid, sensitive, and precise detection and quantification of various RNA sequences via hydrolysis probes. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.
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PerfeCTa® qPCR FastMix® II
Supplier: Quantabio
PerfeCTa® qPCR FastMix® II is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR FastMix® II is provided as a 2X concentrated ready to use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.
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VWR® Probe One-Step RT-qPCR Master Mix with UDG
Supplier: VWR Chemicals
VWR® Probe One-Step RT-qPCR Master Mix with UDG is designed for the detection and quantitation of RNA targets and is an ideal choice for multiplex applications. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.
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VWR® Probe qPCR Master Mix
Supplier: VWR Chemicals
VWR® Probe qPCR Master Mix features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.
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VWR®, Real-time PCR kit for Legionella spp. (ISO/TS 12869)
Supplier: VWR Chemicals
Real-Time PCR kit for detection and quantification of Legionella spp. in environmental samples (ISO/TS 12869). Kit contains all necessary reagents for 100 reactions. This product provides rapid and efficient detection and quantification of Legionella spp.
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VWR®, Real-Time PCR kit for Salmonella spp. in food samples
Supplier: VWR Chemicals
Real-Time PCR kit for detection and quantification of Salmonella spp. in food samples. Kit that contains all necessary reagents for 100 reactions. This product provides rapid and efficient detection and quantification of Salmonella spp.
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VWR® qPCR Master Mix
Supplier: VWR Chemicals
VWR® qPCR Master Mix features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.
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VWR®, Real-time PCR kit for Listeria Monocytogenes
Supplier: VWR Chemicals
This kit is designed to detect the pathogen microorganism Listeria monocytogenes in food samples, after enrichment steps, according to standard microbiology procedures. It can also be used to confirm suspect colonies isolated in selective culture media.
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qPCR SuperMixes, PerfeCTa®
Supplier: Quantabio
PerfeCTa® qPCR SuperMixes are 2X concentrated, ready to use reaction cocktails containing all components except primers, probe and template for qPCR.
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MVP human total RNA
Supplier: AGILENT
MVP RNA is application-ready RNA available in small economical pack sizes.
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Fast probe master mixes
Supplier: Biotium
A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.
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AMPIGENE® qPCR 1-Step Green Kit Hi-ROX
Supplier: ENZO LIFE SCIENCES
Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.
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PerfeCTa® MultiPlex qPCR ToughMix®
Supplier: Quantabio
PerfeCTa® MultiPlex qPCR ToughMix® is a 5X concentrated, ready to use reaction cocktail for Real-Time quantitative PCR (qPCR) that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers, probes and templates. The 5X concentrated ToughMix® allows addition of higher amounts of template and improved detection sensitivity with low concentration samples. PerfeCta® MultiPlex qPCR ToughMix® has been optimised to deliver maximum PCR efficiency, sensitivity, and specificity in reduced reaction volumes with fast cycle or conventional PCR cycling protocols.
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Ultra-Plex™ 1-Step ToughMix® (4X)
Supplier: Quantabio
Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.
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One-Step SYBR® Green qRT-PCR, qScript™
Supplier: Quantabio
qScript™ One-Step SYBR® Green qRT-PCR Kits reliably deliver maximum qRT-PCR efficiency, sensitivity and specificity in with a simplified protocol. These kits offer reliable and unbiased amplification of all RNA targets regardless of abundance. The proprietary supermix maximises activities of both reverse transcriptase and AccuStart™ Taq DNA polymerase without compromising specificity of amplification. qScript™ One-Step SYBR® Green qRT-PCR kits are available and optimised for all real-time PCR instrument platforms including those requiring normalisation with ROX reference dye.
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Brilliant III Ultra-Fast SYBR® green ROX QPCR master mix
Supplier: AGILENT
High performance, ultra-sensitive, SYBR® Green QPCR master mix reagent with ROX concentration for reliable quantification across a wide range of targets and templates.
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AMPIGENE® qPCR 1-Step Probe Kit Lo-ROX
Supplier: ENZO LIFE SCIENCES
Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.
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qPCR master mixes, Forget-Me-Not™ EvaGreen®
Supplier: Biotium
EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.
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qPCR master mixes, Fast Plus EvaGreen®
Supplier: Biotium
A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.
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PerfeCTa® QPCR mixes, SYBR® green super mixes and fast mixes
Supplier: Quantabio
PerfeCTa® SYBR® Green SuperMixes and FastMixes™ enable efficient, sensitive and precise quantitative PCR with proprietary buffers and SYBR® Green stabilisers that maximise fluorescent signal, PCR efficiency, and reduce primer dimers. These SuperMixes and FastMixes™ have been optimised for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye or fluorescein.
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Brilliant III Ultra-fast probe ROX qPCR master mix
Supplier: AGILENT
High performance, ultra-sensitive, probe QPCR master mix reagent with high or low ROX concentration for reliable quantification across a wide range of targets and templates.
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Viability PCR starter kits
Supplier: Biotium
Kits designed to make it simple to start performing viability PCR with the choice of viability dye (PMA or PMAxx™).
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Taq extender PCR additive
Supplier: AGILENT
Improves reliability and yield of conventional Taq-based PCR amplifications.
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One-Step Fast qRT-PCR kits, qScript™
Supplier: Quantabio
These kits offer a convenient and highly sensitive solution for Real-Time reverse transcriptase PCR (qRT-PCR) of RNA templates using hybridisation probe detection chemistries, such as TaqMan® 5'-hydrolysis probes.
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AMPIGENE® qPCR 1-Step Probe Kit No-ROX
Supplier: ENZO LIFE SCIENCES
Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.
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Virus detection kit, qScript™ 1-Step Virus ToughMix®
Supplier: Quantabio
Superior sensitivity for viral RNA detection.