Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

Succinate, 0,2 M buffer solution, pH 5,0

Supplier: Thermo Fisher Scientific

Succinate, 0.2M buffer soln., pH 5.0, Thermo Fisher Scientific

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Triton® X-100 lysis buffer with glycerol (2X), pH 7,4

Supplier: Thermo Fisher Scientific

Triton® X-100 lysis buffer with glycerol (2X), pH 7.4, Thermo Fisher Scientific

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ACK Lysing Buffer (1x), TheraPEAK™

Supplier: LONZA

Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used to lyse red blood cells in preparations containing white blood cells.

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GTE buffer

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific

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MNE buffer, pH 6,5

Supplier: Thermo Fisher Scientific

MNE buffer, pH 6.5, Thermo Fisher Scientific

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NP-40 lysis buffer, low salt

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer, low salt, Thermo Fisher Scientific

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HEPES lysis buffer with NP-40 (2X)

Supplier: Thermo Fisher Scientific

HEPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific

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Cell lysis buffers

Supplier: Rockland Immunochemicals

Cell lysis buffer is used to lyse cells under non-denaturing conditions.

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Protein extraction reagent, BugBuster® HT, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

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G-Biosciences ACD (Acid Citrate Dextrose) Solutions for Anticoagulants

Supplier: G-Biosciences

ACD (Acid Citrate Dextrose) Solution A and ACD (Acid Citrate Dextrose) Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma.