Supplier: G-Biosciences

This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.

Supplier: G-Biosciences

The Physical Properties of Proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

Supplier: EDVOTEK

In this PCR experiment, students make billions of copies of a small amount of DNA in only 90 minutes. They just need to mix template DNA and primers with PCR beads that contain all of the other components required to carry out a PCR reaction. Students see the increasing amounts of DNA for themselves, taking samples every few cycles and analysing them on a DNA gel.

Supplier: EDVOTEK

In DNA fingerprinting, variable number tandem repeats (VNTR) are used to identify individuals. Using this kit, students type themselves at the D1S80 locus on chromosome 1. This region contains between 14 and 40 copies of a 16 base pair repeat.

Supplier: EDVOTEK

Students can solve a crime using PCR. Plasmid DNA is provided that, when amplified by PCR, provides products that represent individual DNA profiles.

Supplier: EDVOTEK

This simulation experiment demonstrates the process of DNA amplification by PCR and how the amplified product is detected by separating the reaction mixture by agarose gel electrophoresis.

Supplier: EDVOTEK

Mit dem Ready-to-Load™ PCR-Kit werden Studenten in die Prinzipien und Anwendungen der Polymerasekettenreaktion (PCR) eingeführt. Das Simulationsexperiment enthält keine Human-DNA und erfordert keinen Thermocycler.

Supplier: EDVOTEK

This experiment uses PCR to amplify a small section of Lambda DNA via a 2-step process, saving valuable classroom time and allowing completion of the lab in one session.

Supplier: G-Biosciences

Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.

Supplier: EDVOTEK

Students use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype. They can then compare their class results with others around the world over the internet.

Supplier: EDVOTEK

Anhand dieses einfachen und ungefährlichen Experiments erhalten die Studenten einen Einblick in die Enzymkatalyse, die Merkmale der Enzymaktion sowie die Beziehung zwischen Proteinstruktur und -funktion. Die Studenten führen einen Enzym-Assay durch und bestimmen die Rate der enzymatischen Reaktion.

Supplier: EDVOTEK

Solve the mystery of two boys separated from their parents a decade ago. Their biological mother is identified by mitrochondrial DNA and their biological father from chromosomal DNA.

Supplier: EDVOTEK

The mitochondria are thought to have evolved from a symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, students amplify two regions of their mitochondrial DNA.

Supplier: EDVOTEK

Anhand von Gentests können Menschen mit einer erblich bedingten Erkrankung erkannt werden, die zu einem erhöhten Cholesterinspiegel führt und tödlich ausgehen kann. Studenten sehen, wie ein Gentest durchgeführt wird, und lernen die DNA-Elektrophorese kennen.

Supplier: EDVOTEK

Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.

Supplier: EDVOTEK

Die schnelle Isolierung von Plasmid-DNA in kleinem Maßstab ist ein Routineverfahren für das Screening und die Analyse rekombinanter DNA-Moleküle in Klonierungs- und Subklonierungsversuchen. In diesem Experiment isolieren die Studenten Plasmid-DNA ohne Zuhilfenahme von giftigen Chemikalien wie Phenol oder Chloroform.

Supplier: EDVOTEK

Plasmid- und Lambda-DNA werden mit Restriktionsendonukleasen vorverdaut, die doppelsträngige DNA innerhalb oder in der Nähe definierter Basensequenzen erkennen und zerschneiden. Die Verdauungsprodukte werden mittels Agarose-Gelelektrophorese getrennt.

Supplier: EDVOTEK

In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the lacZ gene. IPTG is not required in this experiment since pGAL contains the complete lacZ gene.

Supplier: EDVOTEK

Learn the basics of DNA sequencing and sequence homology with this lab activity.

Supplier: EDVOTEK

Dieses Ready-to-Load™ Kit zeigt, wie ein Restriktionsenzym zum Screening der DNA für Sichelzellanämie verwendet werden kann.

Supplier: EDVOTEK

Experiment using fluorescence and electrophoresis to solve the mystery of the invisible bands.

Supplier: EDVOTEK

Zur Erläuterung der Vielfalt von Proteinen durch Untersuchung der elektrophoretischen Profile verschiedener Quellen. Studenten separieren Proteine von Bakterien, Pflanzen, Serum und Milch und vergleichen diese mit einem Standard-Proteinmarker.

Supplier: EDVOTEK

The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for Eco RI (5 distinct sites; 2 are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.

Supplier: EDVOTEK

In this experiment, students study the diversity of fish using pre-stained, lyophilised proteins. Total protein from perch, walleye and salmon is extracted and pre-stained using an indicator dye. Each fish protein sample has a characteristic banding pattern when separated by denaturing SDS-polyacrylamide gel electrophoresis, which can be used to identify the specific species.

Supplier: EDVOTEK

Dieses Experiment stellt ein Bioterrorismus-Szenario vor, wobei die Studenten einen simulierten genetischen Fingerabdrucktest zur Erkennung von Pocken untersuchen.

Supplier: EDVOTEK

Kit allows students to construct DNA models and then extract DNA from onions, strawberries or bananas.

Supplier: EDVOTEK

Mit diesem Kit lernen Studenten, wie die körperlichen Eigenschaften eines Menschen seine Gene reflektieren. In dieser Simulation trennen Studenten mittels Elektrophorese Farbstoffe auf, die erbliche Eigenschaften darstellen.

Supplier: EDVOTEK

In this experiment, students determine a pedigree for a family thought to be carriers of a mutation in their p53 genes. This is followed by a diagnostic agarose gel analysis to diagnose the state of the p53 gene in individual family members.

Supplier: EDVOTEK

This experiment helps teach students about restriction enzyme digests in the context of forensic science. Students cut DNA with restriction enzymes and then compare the banding pattern of the crime scene DNA versus that of two suspects using agarose gel electrophoresis.

Supplier: EDVOTEK

Dieses einfache Experiment demonstriert die Detektion der Mutation, die Sichelzellanämie verursacht. In dieser Simulation trennen die Studenten mittels Elektrophorese Farbstoffe auf, die Patientenproben und Kontrollen darstellen.