Supplier: SIGMA ALDRICH MICROSCOPY

Histo/Zyme is a stabilized proteolytic enzyme in buffered solution, used to unmask immunoreactive sites altered by fixation and/or the embedding process. Treatment with a proteolytic enzyme in immunohistochemistry (IHC) is required to digest excess linkages and expose the antigen. The use of Histo/Zyme results in superior staining over traditionally used enzyme pretreatments, such as pepsin and trypsin. Moreover, Histo/Zyme requires only a brief incubation at room temperature. Performing antigen recovery with Histo/Zyme will allow you to dilute the primary antibody 5 to 10 fold further and achieve optimal staining.

Supplier: VWR Chemicals

Pepsin is a proteolytic enzyme product derived from porcine stomach linings.

Supplier: Merck

Pronase E is a mixture of proteolytic enzymes that is obtained from Streptomyces griseus. It can be used in the in diagnostics in histopathology as it hydrolyses proteins into individual amino acids.

Supplier: Thermo Fisher Scientific

Yeast lytic enzyme

Supplier: VWR Chemicals

10,000 units is that quantity of enzyme which will degrade 2 cm² of cellulose filter paper (17 mg) in 1 minute in 0,1 M acetate buffer at pH 4,0.

Supplier: Cayman Chemical

An active, pure human recombinant enzyme.

Supplier: ENZO LIFE SCIENCES

Isolated from human neutrophils. Active enzyme.

Supplier: Cayman Chemical

An active, pure human recombinant enzyme.

Supplier: Merck

Yeast invertase

Supplier: Merck

Proteinase K (from tritirachium album) solution in tris/HCl for molecular biology.

Supplier: SIGMA ALDRICH MICROSCOPY

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Supplier: ENZO LIFE SCIENCES

Produced in E. coli. The enzyme is cleaved and activated from the proenzyme.

Supplier: VWR Chemicals

Uracil-DNA glycosylase (UNG), cod is a thermolabile recombinant enzyme produced in E. coli (ung-) using a modified ung gene derived from Atlantic cod. It degrades uracil-containing single- and double-stranded DNA, but not RNA or thymidine-containing DNA, by hydrolysing the N-glycosidic bond between deoxyribose sugar and the base in uracil. This generates alkaline-sensitive apyramidinic sites in the DNA that will be cleaved upon a combination of alkaline conditions and high temperature.

Supplier: Novus Biologicals

Lipo protein lipase (LPL) is a rate-limiting enzyme responsible for the hydrolysis of triglycerides.

Supplier: Merck

Tritirachium album proteinase K

Supplier: ProSci Inc.

Sulfotransferase 1C2 (SULT1C2) is a cytosolic enzyme member of the Sulfotransferase 1 family.

Supplier: ProSci Inc.

Alpha-amylase 2B is an enzyme that in humans is encoded by the AMY2B gene.

Supplier: Avantor

J.T.Baker® Endonuclease Ultrapure Bioreagent is designed for the degradation of both single stranded and double stranded DNA and RNA. J.T.Baker® Endonuclease Ultrapure Bioreagent is used to ensure host cell DNA impurities are removed; driving process efficiency by lowering viscosity and preventing aggregation. J.T.Baker® Endonuclease is an enzyme based upon the native endonuclease of Serratia marcescens, enabling rapid clearance of residual DNA and RNA during the production and purification of both recombinant proteins and viral vectors. Non-animal origin. The purity of materials in non-negotiable. J.T.Baker® Endonuclease Ultrapure Bioreagent acts to degrade and eliminate extraneous genetic material, ensuring the pristine quality of your final product.

Supplier: Thermo Fisher Scientific

Agarase is an enzyme that specifically digests the agarose polysaccharide core into neoagaro-oligosaccharides.

Supplier: Merck

Urease is used for biochemical research and analysis.

Supplier: Merck

Aldehyde dehydrogenase (from Yeast)

Supplier: ENZO LIFE SCIENCES

Produced in E. coli. Tyrosinase is a copper-containing enzyme that catalyzes four steps in the melanin and catecholamine synthesis pathways.

Supplier: US Biological

DNA topoisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription.

Supplier: Merck

Papain

Supplier: Cayman Chemical

Thrombin is the last enzyme in the clotting cascade functioning to cleave fibrinogen to fibrin and potentiates the procoagulation process by activating factors V, VIII, XI, and XIII.

Supplier: Merck

Diastase (from fungi)

Supplier: ProSci Inc.

Pyruvate kinase isoform M2 (PKM2) is a member of the pyruvate kinase (PK) family, a pivotal glycolytic enzyme that is consistently changed during tumorigenesis.

Supplier: Merck

Oxidase used for the detection of cytochrome oxidase in microorganisms.

Supplier: SIGMA ALDRICH MICROSCOPY

Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. Pepsin digestion of FFPE tissue sections improves accessibility of antibodies to tissue antigens. Ready to use pepsin reagent, antigen retriever has been used for antigen unmasking in immunohistochemistry.

Supplier: Cayman Chemical

Riboflavin 5'-monophosphate (FMN) is a coenzyme that is tightly bound to enzymes catalysing oxidation and reduction reactions in a variety of biosynthetic pathways.