Technische Daten
- VE:96 Tests
- Testdauer:Multiple steps
- Testtyp:Sandwich
- Format:Pre-coated
- Reaktivität der primären Antikörper:Human
- Target protein:Carbonic Anhydrase 14/CA14
- Bezeichnung:Human Carbonic Anhydrase 14/CA14 ELISA kit
- Probentyp:Serum, plasma, tissue homogenates and other biological fluids
- Kreuzreaktivität:Human Carbonic Anhydrase 14/CA14 ELISA kit exhibits high specificity and excellent specificity for the detection of human Carbonic Anhydrase 14/CA14. No significant cross-reactivity or interference between Carbonic Anhydrase 14/CA14 and analogues was observed.
- Erkennungsmethode:Colorimetric
- Time to Results:4 h 30 min
- Testprinzip:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Erfassungsbereich:0,156 - 10 ng/ml
- Lagertemperatur:4 °C
- Probenvolumen:100 μl
- Empfindlichkeit:0,094 ng/ml
- Regulatory status:RUO
Technische Daten
Über diesen Artikel
Human Carbonic Anhydrase 14/CA14 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Carbonic Anhydrase 14/CA14 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 0,156 to 10 ng/ml
- Sensitivity: 0,094 ng/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra assay: CV <8%, Inter assay: CV <10%
Human Carbonic Anhydrase 14 / CA14 ELISA kit (A77786) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Carbonic Anhydrase 14 / CA14 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Carbonic Anhydrase 14 / CA14 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Carbonic Anhydrase 14 / CA14 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Carbonic Anhydrase 14 / CA14 Antibody, which binds the captured Carbonic Anhydrase 14 / CA14 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Carbonic Anhydrase 14 / CA14 captured in each well. The concentration of Carbonic Anhydrase 14 / CA14 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.