buffers
Pufferlösungen sind pH-beständig und schützen Proben bei Zugabe von Säuren oder Laugen. Sie sind als Pulver und Flüssigkeiten erhältlich und ermöglichen die effiziente Verfolgung von Proteinen während der elektrophoretischen Isolierung und Trennung. Salzhaltige Pufferlösungen eignen sich zudem zur Kalibrierung und Standardisierung von pH-Metern für präzise Messwerte. Blockierende Substanzen verhindern die Bindung von Antikörpern an freie Membranstellen, wodurch ein störender Hintergrund entstehen würde. Die Pufferlösungen sind in verschiedenen Konzentrationen, sowohl flüssig als auch dehydriert, erhältlich und decken somit jeden Bedarf ab.
Tris-Glycine native gel running buffer
Supplier: G-Biosciences
Laufpuffer, Tris-Glycine native gel running buffer
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10X Blocking Buffer, Abcam
Supplier: Abcam
10X blocking buffer (pH 7.5) for serum-free blocking of non-specific antibody binding. - Cited in over 30 publications - Suitable for ELISA, in-cell ELISA, ICC/IF, western blot (WB) - 100% casein blocking as preservative
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Extraction Buffer 5X PTR, Abcam
Supplier: Abcam
Extraction buffer 5X PTR is formulated for preparing cell or tissue extracts for use in immunoassays, with added phosphatase inhibitors and protease inhibitor aprotinin. - Cited in over 20 publications - Phosphatase and protease inhibitors – contains inhibitors to preserve sample integrity - Easy to use – dilute to 1X with distilled water before use, or use with extraction enhancer buffer 50X for enhanced extraction - Compatible with a variety of sample types
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Protein solubilisation buffer
Supplier: G-Biosciences
FOCUS™ protein solubilisation buffer is a dry, urea-based buffer for protein solubilisation. It is a pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulphobetaine for maximum solubilising strength.
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10X Wash Buffer PT
Supplier: Abcam
10X Wash Buffer PT (ab206977) is part of the reagents, controls and accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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TM Buffer, 1X Tris-Magnesium sulphate, pH 7,5
Supplier: Rockland Immunochemicals
This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 50 mM Tris HCl, 10 mM magnesium sulphate at pH 7,5 and was prepared using ultra pure reagents dissolved in pharmaceutical grade deionised water.
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In-situ hybridisation buffer (1.25X concentrate), Enzo Life Sciences
Supplier: Enzo Life Sciences
In Situ Hybridization Buffer provides a high quality hybridization medium for in situ hybridization analyses using either DNA or oligonucleotide probes labeled with biotin-, digoxigenin- or fluorescein-modified nucleotides.
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10X RIPA Buffer, Abcam
Supplier: Abcam
10X RIPA lysis buffer (pH 7.5) is a means of cell lysis and protein solubilization for both adherent and suspension-cultured mammalian cells. - Cited in over 180 publications - Suitable for immunoprecipitation (IP), ELISA, SDS-PAGE, western blot - Effectively extracts cytoplasmic, nuclear and membrane proteins
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Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0), Abcam
Supplier: Abcam
Antigen retrieval 100X tris-EDTA buffer (pH 9.0) enables target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. - Cited in over 20 publications - Contains 5% tris, 5% tetrasodium ethylene diamine tetraacetate, 1.5% 2-butoxyethanol - Optimized for tris-EDTA buffer (pH 9.0) treated antibodies - Contains detergent for paraffin emulsification
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Tissue Clearing Permeabilization Buffer - hydrophobic, Abcam
Supplier: Abcam
Tissue Clearing Permeabilization Buffer ab243307 is part of our range of tissue clearing reagents and products that enable you to set up tissue clearing easily and quickly.
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VWR® UltraPure, Phosphate buffered saline (PBS), powdered
Supplier: VWR Chemicals
PBS, Ultra Pure Grade is a powdered, magnesium- and calcium-free formulation of PBS suitable for cell culture and for use in flow cytometry applications.
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pH buffer solutions
Supplier: Hach
pH buffer standard solution with certificate of analysis, 4×250 ml, pH-Wert: 7,00, Plastikflasche
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ReadiUse™ Cell Detaching Buffer (50 ml)
Supplier: AAT Bioquest
ReadiUse™ cell detaching buffer is a cell detachment solution that does not contain mammalian or bacterial-derived products.
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VWR® Buffer, MES-SDS, 20X, UltraPure Grade
Supplier: VWR Chemicals
Genutzt für hohe Auftrennleistung von Proteinen in pH-neutralen SDS-PAGE Gelen.
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Electrophoresis running buffers, Pierce™
Supplier: Thermo Fisher Scientific
These are space-saving stock solution that is ideal for quickly preparing running buffer used for gel electrophoresis.
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Tris Buffers, Sterile
Supplier: G-Biosciences
Tris is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane, with the formula (HOCH2)3CNH2. Tris is extensively used in as a major component in buffer solutions.
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