buffers

Pufferlösungen sind pH-beständig und schützen Proben bei Zugabe von Säuren oder Laugen. Sie sind als Pulver und Flüssigkeiten erhältlich und ermöglichen die effiziente Verfolgung von Proteinen während der elektrophoretischen Isolierung und Trennung. Salzhaltige Pufferlösungen eignen sich zudem zur Kalibrierung und Standardisierung von pH-Metern für präzise Messwerte. Blockierende Substanzen verhindern die Bindung von Antikörpern an freie Membranstellen, wodurch ein störender Hintergrund entstehen würde. Die Pufferlösungen sind in verschiedenen Konzentrationen, sowohl flüssig als auch dehydriert, erhältlich und decken somit jeden Bedarf ab.

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SSPE Buffer, 20X concentrate, pH 7,4

Supplier: Rockland Immunochemicals

20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Supplier: Thermo Fisher Scientific

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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Cell Lysis Buffer, Abcam

Supplier: Abcam

This product is manufactured by BioVision, an Abcam company and was previously called 1067 Cell Lysis Buffer.

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RNA isolation buffers

Supplier: OMEGA BIO-TEK

RNA Wash Buffer I

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Supplier: PanReac AppliChem

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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G-Biosciences TBE Running Buffer (10X)

Supplier: G-Biosciences

The primary use of TBE running buffer is in agarose electrophoresis for the separation of nucleic acids (DNA and RNA).

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AEC Substrate Buffer, Abcam

Supplier: Abcam

This product is manufactured by BioVision, an Abcam company and was previously called 2123 AEC Substrate Buffer.

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EDTA Buffer, pH 8,5, 10X, Antigen Retriever

Supplier: SIGMA ALDRICH MICROSCOPY

Used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

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HEPES Buffer Solution 1 M, HyClone™

Supplier: HyClone products (Cytiva)

Maintain physiological pH in cell culture, despite changes in carbon dioxide concentration (produced by cellular respiration) with Cytiva HyClone HEPES solution.

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Antigen Retrieval Buffer (100X Citrate Buffer), Abcam

Supplier: Abcam

Antigen retrieval 100X citrate buffer (pH 5.1 - 5.5) is a special solution that enables rehydration and antigen retrieval to be performed in formalin-fixed, paraffin-embedded tissue sections with one step. - Cited in over 25 publications - Contains citrate buffer, 1.5% 2-butoxyethanol, 1% hydrochloric acid - Optimized for citrate buffer-treated antibodies - Contains detergent for paraffin emulsification

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Cell Lysis Buffer, Abcam

Supplier: Abcam

ab152163 is a 10X Cell Lysis Buffer used to lyse cells under non-denaturing conditions.

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Native sample loading buffer, 2X

Supplier: G-Biosciences

Loading Buffer, Native sample loading buffer (2X)

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Native lysis Buffer, Abcam

Supplier: Abcam

Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.