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buffers

Puffer

Pufferlösungen sind pH-beständig und schützen Proben bei Zugabe von Säuren oder Laugen. Sie sind als Pulver und Flüssigkeiten erhältlich und ermöglichen die effiziente Verfolgung von Proteinen während der elektrophoretischen Isolierung und Trennung. Salzhaltige Pufferlösungen eignen sich zudem zur Kalibrierung und Standardisierung von pH-Metern für präzise Messwerte. Blockierende Substanzen verhindern die Bindung von Antikörpern an freie Membranstellen, wodurch ein störender Hintergrund entstehen würde. Die Pufferlösungen sind in verschiedenen Konzentrationen, sowohl flüssig als auch dehydriert, erhältlich und decken somit jeden Bedarf ab.

 
 

Caspase assay buffer

Supplier: Enzo Life Sciences

50 mM HEPES, pH 7,4, 100 mM NaCl, 0,1% CHAPS, 10 mM DTT, 1 mM EDTA, 10% glycerol.

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Cell culture salts, salt solutions and buffers

Cell culture salts, salt solutions and buffers

Supplier: Biowest

These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

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North2South™ Hybridisation Buffer

North2South™ Hybridisation Buffer

Supplier: Thermo Fisher Scientific

North2South™ hybridisation buffer is an aqueous buffer which is used with the North2South™ chemiluminescent detection kit.

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Permeabilization Buffer (Flow Cytometry), Abcam

Supplier: Abcam

Permeabilization Buffer (ab314681) is a ready to use permeabilization buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.

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Competent cell buffer

Supplier: Rockland Immunochemicals

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 60 mM Calcium Chloride, 15% v/v Glycerol, 10mM Tris HCl at a pH of 7,5.

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VWR®, Buffers, Sodium acetate

VWR®, Buffers, Sodium acetate

Supplier: VWR Chemicals

Sodium acetate buffer B, VWR®

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G-Biosciences Sodium Chloride-Sodium Phosphate-EDTA Buffers (20X)

G-Biosciences Sodium Chloride-Sodium Phosphate-EDTA Buffers (20X)

Supplier: G-Biosciences

Saline-sodium phosphate-EDTA hybridisation buffer [20X].

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Fixation Buffer (Flow Cytometry), Abcam

Supplier: Abcam

Fixation Buffer (ab314680) is a ready-to-use formaldehyde-based fixation buffer for immunofluorescence cell staining for microscopy or flow cytometry.

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Cell lysis buffer, RIPA

Supplier: Enzo Life Sciences

The RIPA liquid cell lysis buffer composes of 50 mM TRIS-hydrogen chloride, pH 7,4, containing 150 mM sodium chloride, 1 mM EDTA, 1 mM EGTA, 1,2% Triton X-100, 0,5% sodium deoxycholate, and 0,1% SDS.

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Ionic strength adjustor buffer solution for fluoride electrode, Supelco®

Supplier: Merck

It may be used as TISAB buffer in determination of fluoride in coal, coal fly ash, phosphate rock, limestone, mineral clay, fossilised materials, oyster tissue and vegetation using pyrohydrolysis for sample decomposition.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/l)

Supplier: G-Biosciences

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0), Abcam

Supplier: Abcam

Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step.

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Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0), Abcam

Supplier: Abcam

Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step.

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In-situ hybridisation buffer for HPV probes

Supplier: Enzo Life Sciences

In situ hybridisation buffer for HPV probes (ready-to use) provides a high quality hybridisation medium for In situ hybridisation analyses using either DNA or oligonucleotide probes labeled with biotin-, or fluorescein-modified nucleotides.

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