buffers
Pufferlösungen sind pH-beständig und schützen Proben bei Zugabe von Säuren oder Laugen. Sie sind als Pulver und Flüssigkeiten erhältlich und ermöglichen die effiziente Verfolgung von Proteinen während der elektrophoretischen Isolierung und Trennung. Salzhaltige Pufferlösungen eignen sich zudem zur Kalibrierung und Standardisierung von pH-Metern für präzise Messwerte. Blockierende Substanzen verhindern die Bindung von Antikörpern an freie Membranstellen, wodurch ein störender Hintergrund entstehen würde. Die Pufferlösungen sind in verschiedenen Konzentrationen, sowohl flüssig als auch dehydriert, erhältlich und decken somit jeden Bedarf ab.
Buffer HF-Improved
Supplier: Thermo Fisher Scientific
Buffer used for pretreatment of planar silicon devices when plating with nickel plating solution products 44069 and 44070.
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Dry buffer packs, JAW™
Supplier: G-Biosciences
Premixed buffer powders that simply require adding water to generate a ready-to-use buffer.
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Proteasome assay buffer
Supplier: Enzo Life Sciences
Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.
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MMP-1 colorimetric assay buffer
Supplier: Enzo Life Sciences
MMP-1 colorimetric assay buffer
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TE Buffer (1X) pH 8,0 for Molecular Biology
Supplier: Apollo Scientific
Cloning Buffer, TE buffer (1X)
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Hybridisation buffers
Supplier: G-Biosciences
The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).
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Wash buffer concentrate
Supplier: Enzo Life Sciences
Liquid. TRIS buffered saline containing detergents.
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IHC Wash Buffer, Enzo Life Sciences
Supplier: Enzo Life Sciences
The IHC wash buffer is a for washing and removing excess reagents during immunostaining.
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Gram Negative lysis buffer
Supplier: G-Biosciences
Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.
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Wash buffer salts, lyophilised, Enzo Life Sciences
Supplier: Enzo Life Sciences
Wash Buffer Salts can be used in tissue preparations, immunohistochemical staining procedures and in assays that require HRP-linked detection reagents.
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Protein Transfer buffers
Supplier: G-Biosciences
Efficient™ Western Transfer Buffer (20X) achieves increased protein transfer efficiency. This ready-to-use transfer buffer is prepared for optimal conductivity and efficient protein transfer, without generating excessive heat or transfer distortion.
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Fixation buffer for microscopy and flow cytometry
Supplier: Biotium
Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.
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RIPA cell lysis buffer 2
Supplier: Enzo Life Sciences
RIPA cell lysis buffer 2, Enzo Life Sciences
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Optimizer Buffer™ for optimal cross-linking and modification
Supplier: G-Biosciences
The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.
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Lithium nitrate, ionisation buffer solution, Specpure®
Supplier: Thermo Fisher Scientific
Lithium Nitrate Buffer Solution
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