1070 Ergebnisse für: "SIGMA ALDRICH MICROSCOPY"

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Immersion oil is used for high resolution (1000X) light microscopy work under oil immersion objective lens.

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2',4'-Dihydroxy-3'-methylacetophenon 90%, technische Qualität, Sigma-Aldrich®

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N-Benzylidenebenzenesulfonamide,97%

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Bromchlorphenolblau 95% (Farbstoffgehalt), Sigma-Aldrich®

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Tetrabromophenol Blue sodium salt, Dye content 85% (3,3',3'',4,5,5',5'',6-Octabromophenolsulfonphthalein sodium salt).

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3,6-Dibutoxy-1,2-benzenedicarbonitrile,97% (3,6-Dibutoxyphthalonitrile)

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Tetramethylrhodamine isothiocyanate Isomer R (TRITC) is the R isomer of tetramethylrhodamine isothiocyanate and is also known as 6-tetramethylrhodamine isothiocyanate. It is a strongly fluorescent compound soluble in ethanol, methanol, dimethylformamide, dimethylsulfoxide, and water. TRITC is a moderately-sized aminoxanthene dye carrying carboxylic acid and isothiocyanate substituents on the non-planar pendant phenyl ring. It forms a lipophilic cation in acidic pH and a hydrophilic zwitterion around neutral pH. The isothiocyanate group reacts with nucleophilic substituents (e.g., amino, hydroxyl, thiol) of biomolecules, providing the means of attaching a fluorescent label. TRITC also reacts with water and hydroxide ions and is unstable in aqueous solutions. TRITC is commercially available as a zwitterion or as a chloride.

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10% Neutral buffered formalin (NBF) is the most commonly used fixative for light microscopy. 10% NBF is well suited for large throughput laboratories and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship.

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Organo/Limonene Mount™ is a ready-to-use mounting medium is made with limonene, a natural product from orange peels. It is good for coverslipping stained tissues and cell smears that can be dehydrated with organic solvents.

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p-Coumaric acid has been used as a component of chemiluminescent substrate for protein detection in western blotting.

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Siliconizing reagent for glass and other surfaces. Sigmacote® is a solution of a chlorinated organopolysiloxane in heptane that readily forms a covalent, microscopically thin film on glass.

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Fluorescein 95% (Farbstoffgehalt), Sigma-Aldrich®

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4-Chloro-7-sulphobenzofurazan ammonium salt, Pulver

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Fast blue RR salt has been used in the immunohistochemistry to detect the activity of alkaline phosphatase. It has been used in the procedure of connective tissue staining using picrosirius red.

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N,N-Dimethyl-4-nitrosoaniline has been used along with imidazole to detect the formation of singlet oxygen in cells.

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Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

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Lichtgrün SF gelblich, Sigma-Aldrich®

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This kit is intended for demonstration of nonspecific esterase in a subset of lymphocytes for determining maturation arrest. Kits 90A1 or 91A are intended for monocyte identification.

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Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.
Crystal violet solution has been used as a stain in cell proliferation assays, migration assays and Boyden chamber assay.

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Tissues are stained for Gram-positive organisms with crystal violet, mordanted in iodine, decolorized, stained for Gram-negative organisms with safranin O and then counterstained with tartrazine. For delineating Gram-positive and Gram-negative organisms

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Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

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Tetramethylrhodamine-5-isothiocyanate (5-TRITC), Sigma-Aldrich®

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Fluorescent diazoalkane useful as an HPLC derivatization reagent for carboxylic acid detection with better detection limit parameters and greater chemical stability than the commonly used 9-anthryldiazomethane.

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Stains-All has been used for the staining of gel made for size determination of hyaluronan. It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.

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Bromobimane is also known as monobromobimane. It is also a known probe for thiols and is a fluorescent reagent activated upon a photolysis reaction.

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Methyl Blue is a triaminotriphenylmethane dye, widely used in paper, textile and leather production.

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General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.

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ACCUSPIN System-Histopaque-1077 products are intended for use in the isolation of lymphocytes and other mononuclear cells. The separation medium, Histopaque-1077, is a sterile-filtered, endotoxin-tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1,077 g/ml. ACCUSPIN System-HISTOPAQUE-1077 employs γ-irradiated 12mL polypropylene centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ('frit'). Each tube is aseptically filled with 3 mL of Histopaque 1077. Each tube will separate 3 to 6 ml of anticoagulated blood.

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Aurintricarboxylic acid ammonium salt has been used as a nuclease inhibitor to study its effects on transfection.

Supplier: SIGMA ALDRICH MICROSCOPY

May be used as a substitute for trypan blue for some biological and staining applications.