552 Results for: "Merck Millipore (Novagen)"

Supplier: Merck Millipore (Novagen)

A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Supplier: Merck Millipore (Novagen)

The Novagen® BacMagic™-2 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

Supplier: Merck Millipore (Novagen)

The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.

Supplier: Merck Millipore (Novagen)

The Perfect Protein™ HRP Western Blot Kit includes an HRP or AP conjugate in addition to Perfect Protein™ Western markers.

Supplier: Merck Millipore (Novagen)

The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.

Supplier: Merck Millipore (Novagen)

Proteinase K

Supplier: Merck Millipore (Novagen)

Premixed 2X KOD Hot Start PCR components for convenience and reproducibility.

Supplier: Merck Millipore (Novagen)

Der NovaTaq PCR MasterMix ist eine gebrauchsfertige, 2-fach konzentrierte Mischung aus NovaTaq DNS-Polymerase, ultrareinen Desoxynukleotiden und einem Reaktionspuffer ohne Magnesiumchlorid. Der MasterMix erleichtert die Vorbereitung von PCR Reaktionen und bietet die folgenden Vorteile: Zeitersparnis, Konsistenz und minimales Kontaminationsrisiko. Fügen Sie einfach den NovaTaq PCR MasterMix zu einem gleichen Volumen mit den erforderlichen Mengen an Magnesiumchlorid, DNS-Template und Primern hinzu, und die Reaktion ist zum Thermocycling bereit. Die fertig verdünnte Reaktion enthält 2,5 Einheiten NovaTaq DNS-Polymerase pro 100 µl. In der Lieferung sind genug Komponenten für 200 standardmäßige 50-µl-Amplifizierungsreaktionen (bzw. 100×100 µl) enthalten.

Supplier: Merck Millipore (Novagen)

Clonables™ 2X ligation premix is a single solution containing optimised concentrations of T4 DNA ligase, buffer, stabiliser, and cofactors needed for efficient ligation of any type of compatible DNA ends.

Supplier: Merck Millipore (Novagen)

Veggie yeast extract is a certified animal-free media component.

Supplier: Merck Millipore (Novagen)

Molecular size markers for routine use in plasmid cloning, the Perfect DNA™ markers contain sets of DNA species having convenient, easy-to-remember sizes for agarose gel analysis. The markers have uniform band intensities except for the indicated reference bands, which are useful for instant band identification.

Supplier: Merck Millipore (Novagen)

RiboJuice™ siRNA Transfection Reagent delivers siRNA into a wide range of mammalian cell lines for targeted gene suppression. It is compatible with GeneJuice® Transfection Reagent for cotransfection of siRNA and plasmid DNA for normalising transfection efficiency with a reporter gene.

Supplier: Merck Millipore (Novagen)

KOD Xtreme™ Hot Start DNA Polymerase is an ultra high fidelity DNA polymerase designed for the most challenging PCR applications, including crude sample PCR, amplification of long strand and highly GC-rich DNA templates.

Supplier: Merck Millipore (Novagen)

rLysozyme™ solution contains a highly purified and stabilised recombinant lysozyme that can be used for lysis of gram negative bacteria, such as E. coli.

Supplier: Merck Millipore (Novagen)

pET-24(+) is a transcription vector designed for expression from bacterial translation signals carried within a cloned insert. It lacks the ribosome binding site and ATG start codon present on the pET translation vectors. A C-terminal His·Tag® sequence is available

Supplier: Merck Millipore (Novagen)

The pET-24a-d(+) vectors carry an N-terminal T7·Tag® sequence plus an optional C-terminal His·Tag® sequence.

Supplier: Merck Millipore (Novagen)

A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Supplier: Merck Millipore (Novagen)

Die KOD HiFi DNA-Polymerase ist ein einzigartiges Proofreading-Enzym, das aus dem extrem thermophilen Thermococcus kodakaraensis KOD1 isoliert wird (mit höherer Aktivität und Genauigkeit) und somit eine schnellere und genauere PCR-Amplifizierung ermöglicht, als sie mit herkömmlichen Enzymen erreicht werden kann.

Supplier: Merck Millipore (Novagen)

Benzonase® nuclease degrades all forms of DNA and RNA while having no proteolytic activity. Benzonase® is compatible with BugBuster® and PopCulture® protein extraction reagents and therefore can be added to these reagents to eliminate viscosity and remove nucleic acids from E. coli extracts.

Supplier: Merck Millipore (Novagen)

Die Perfect DNA™ Leitern enthalten verschiedene DNA-Arten in praktischen, einprägsamen Größen für die Agarose-Gelanalyse. Die Marker weisen einheitliche Bandenintensitäten auf und decken eine große Bandbreite von DNA-Größen ab.

Supplier: Merck Millipore (Novagen)

Pellet Paint® NF Co-Precipitant ist ein nicht fluoreszierender, mit Farbstoff markierter Träger, der sich für die fluoreszierende Sequenzierung eignet. Er ermöglicht während der Alkoholfällung der Zyklussequenzierung von Reaktionsprodukten die rasche Entfernung von BigDye® Terminatoren. Zyklussequenzierungsreaktionen können mit Zentrifugationszeiten von 10 Minuten schnell gefällt werden. Der gut sichtbare Träger liefert eine einfache Bestätigung, dass die Fällung stattgefunden hat. Die Produkte der Sequenzierungsreaktion werden effizient pelletiert, und die mit Farbstoff markierten Terminatoren verbleiben bei Verwendung der Standard-Fällungsprotokolle von Applied Biosystems während der Alkoholfällung im Überstandmedium. Die Resuspension von pelletierten Sequenzierungsreaktionsprodukten in deionisiertem Formamid kann durch die Überprüfung der Auflösung der Trägerpellets bestätigt werden.

Supplier: Merck Millipore (Novagen)

GST Bind™ Resin utilizes an 14-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of glutathione S-transferase (GST) fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used up to 6 times without loss of capacity.

Supplier: Merck Millipore (Novagen)

The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots, in situ hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant Thermus thermophilus (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

Supplier: Merck Millipore (Novagen)

The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.

Supplier: Merck Millipore (Novagen)

The Mouse Ig-Primer Set is designed for amplification of immunoglobulin (Ig) variable region cDNAs. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR, after which the products may be sequenced directly or cloned.

Supplier: Merck Millipore (Novagen)

The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.

Supplier: Merck Millipore (Novagen)

BugBuster® Plus Lysonase™ includes BugBuster Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimised, ready-to-use blend of rLysozyme™ and Benzonase® Nuclease in a single kit. This powerful combination of two reagents allows maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The BugBuster® and rLysozyme™ efficiently lyse the bacterial cells while the Benzonase® degrades nucleic acids to facilitate downstream processing. Requires 5 ml BugBuster and 10 µl Lysonase per 1 g cell paste. The kits provide sufficient reagents for protein extraction from 20 g or 100 g cell paste.

Supplier: Merck Millipore (Novagen)

D-Tube96™ Dialysers allow convenient, high throughput dialysis of 96 samples simultaneously. The device features the advantages of the D-Tube™ Dialyzer Mini in a 96-tube format. D-Tube Dialysers are easy to handle tubes with dual membranes providing a large surface area for fast, efficient dialysis. The membrane is ultra clean, EDTA-treated, regenerated cellulose that is sulfur and heavy metal-free.

Supplier: Merck Millipore (Novagen)

The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant Taq polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues in vivo following transformation.

Supplier: Merck Millipore (Novagen)

ProteoJuice™ protein transfection reagent is an effective reagent for the transfection of intact functional protein and peptides into mammalian cells with minimal toxicity and broad cell specificity.