"Biowest"

Supplier: Biowest

L-Glutamin ist eine essentielle Aminosäure, die von praktisch allen in Kultur gezüchteten Säugetier- und Insektenzellen benötigt wird. Es ist ein entscheidender Bestandteil vieler Zellkulturmedien und dient als wichtige Energiequelle für Zellen in Kultur. L-Glutamin ist als Trockenpulver und als gefrorene Lösung sehr stabil. In flüssigen Medien oder Stammlösungen kann L-Glutamin jedoch relativ schnell abgebaut werden. L-Glutamin ist labiler in Zellkulturlösungen als andere Aminosäuren.

Supplier: Biowest

Gentamicin sulfate acts against gram-negative, gram-positive bacteria and mycoplasma.

Supplier: Biowest

Dulbecco's Modified Eagle Medium (DMEM) is a modification of Basal Medium Eagle (BME) which contains approximately four times as much of the vitamins and amino acids, and two to four times as much glucose (depending on the version) as the original formulation.

Supplier: Biowest

The long term cultivation of cells requires the addition of serum. When properly supplemented, Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

Supplier: Biowest

Penicillin-Streptomycin acts against Gram-negative and Gram-positive bacteria.

Supplier: Biowest

Lymphosep is a standardised, high quality medium designed for simple and rapid isolation of PBMCs from treated whole blood. Lymphosep, like Ficoll™, has the optimised density 1,077 g/ml for lymphocyte separation to guarantee best and reliable results for your preparations.

Supplier: Biowest

These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

Supplier: Biowest

Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

Supplier: Biowest

In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.

Supplier: Biowest

L(+)-Glutamin 0.2 mol/l in wässriger Lösung

Supplier: Biowest

Colcemid arrests the division of cells in mitosis. It prevents the formation of the spindle apparatus responsible for cell division, thereby permitting an accumulation of metaphases.

Supplier: Biowest

Amphotericin B used in tissue culture to prevent growth of yeasts and fungi. It changes the function and integrity of eucaryotic cell membranes by forming complexes with sterols (cholesterol) hence having no effect on bacteria. Cause leakage of glucose.

Supplier: Biowest

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

Supplier: Biowest

Glutamine penicillin streptamycin acts against gram-negative and gram-positive bacteria.

Supplier: Biowest

Minimum Essential Medium (MEM) with Earle's Balanced Salts is a modification of Eagle's earlier medium, Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle's Balanced Salts, which make them suitable for use in atmospheres charged with CO₂ gas.

Supplier: Biowest

Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.