"Bead Anvil"
Glutathione spin columns, Pierce™
Supplier: Thermo Fisher Scientific
These spin columns provide a fast, easy to use format for purifying GST-fusion proteins from cellular lysates. The glutathione is immobilised through its central sulfhydryl onto 6% crosslinked agarose resin. Purification of GST-fusion proteins using glutathione (GSH) agarose beads is well documented and provides a one-step, high purity affinity purification.
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Cation exchange chromatography columns, TSK-GEL® SP-2SW
Supplier: TOSOH Bioscience
TSK-GEL® SP-2SW are silica based stainless steel column packed with spherical silica beads which are surface modified with sulfopropyl strong cation exchange groups. Designed for the separation and analysis of small protein, peptides, nucleosides and other biologically active molecules.
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Lysing matrix Z, MP Biomedicals
Supplier: MP Biomedicals
Lysing Matrix Z is used primarily for lysis of animal and plant tissue, tougher stems, roots and seeds, and whole insects. Each impact-resistant tube contains yttria-stabilised zirconium oxide beads.
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Immobilised ficin (agarose resin), Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Immobilised Ficin consists of a sulfhydryl-specific protease (ficin) that has been immobilised onto beaded agarose resin to enable controlled antibody fragmentation, especially of mouse IgG1.
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Lysing kits for Fastprep®-24 5G, Lysing Matrix E, MP Biomedicals
Supplier: MP Biomedicals
Each impact-resistant 2 ml tube contains 1,4 mm ceramic spheres, 0,1 mm silica spheres, and one 4 mm glass bead. Lysing Matrix E tubes have purple caps and found in the FastDNA™ SPIN Kit for Soil and the FastRNA™ Pro Soil Kits. Lysing Matrix E can also be effective in lysing mixed samples, such as microbe infected tissue, and can be used in RNA extraction from tumours and other difficult tissues.
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Cation exchange chromatography columns, TSK-GEL® CM-3SW
Supplier: TOSOH Bioscience
TSK-GEL® CM-3SW are silica based stainless steel columns packed with spherical silica beads which are modified with carboxymethyl (CM) ligands to provide a weak cation exchange column. Designed for the separation and analysis of proteins, peptides, and other biologically active molecules.
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Cation exchange chromatography columns, TSK-GEL® CM-5PW
Supplier: TOSOH Bioscience
TSK-GEL® CM-5PW are polymer based stainless steel columns packed with ith porous hydrophilic polymer beads which are surface modified with carboxymethyl (CM) ligands to provide a weak cation exchange column. Used for the separation and analysis of proteins, peptides, and other biologically active molecules.
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Magnetic cell separation nanobeads, CytoSinct™
Supplier: Genscript
Efficient and gentle cell separation based on surface markers is essential for researching specific cell populations, and for developing life-saving cell and gene therapies. GenScript has renowned expertise in developing magnetic bead products for bioseparation of various types of biomolecules. The CytoSinct™ Cell Separation Technology uses perfected nanoparticle empowered immunomagnetic separation technology to isolate specific cell populations of interest, combining the specificity and sensitivity of antibody-based purification with the gentleness of nanoparticles and the ease and speed of magnetic separation.
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Homogeniser, bench top, Fastprep-24 5G™, MP Biomedicals
Supplier: MP Biomedicals
FastPrep®-24 5G is a high speed bench top homogeniser offering the ultimate in speed and performance for the lysis of biological samples. It uses a unique, optimised motion to disrupt cells through the multi-directional, simultaneous beating of specialised Lysing Matrix beads on the sample material. Developed for difficult and resistant samples, the FastPrep®-24 5G instrument lyses thoroughly and quickly any tissues and cells and allows easy and reproducible isolation of stable RNA, active proteins and full-length genomic DNA.
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Control agarose resin, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Control Agarose Resin is underivatised beaded agarose support for pre-clearing protein immunoprecipitation samples.
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Pegylation reagents, methyl-PEG-amine compounds
Supplier: Thermo Fisher Scientific
The methyl-PEGn-amine (MA[PEG]n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.
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Chromatography cartridges, glutathione, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Glutathione chromatography cartridges are convenient, ready to use pre-packed devices for the purification of GST-fusion proteins from cellular lysates. The glutathione is immobilised through its central sulfhydryl onto 6% crosslinked agarose resin. Purification of GST-fusion proteins using glutathione (GSH) agarose beads is well documented and provides a one-step, high purity affinity purification.
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Pierce™ Pegylation Reagents, Carboxy-PEG-Amine Compounds
Supplier: Thermo Fisher Scientific
The carboxy-PEGn-amine (CA[PEG]n) PEGylation reagents are zwitterionic, amino acid derivatives that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.
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Affinity chromatography media, IMAC Sepharose™ 6 Fast Flow
Supplier: Cytiva
IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.
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Filter plates, 96-well, Corning® FiltrEX™
Supplier: Corning
FiltrEX™ filter plates meet the industry standards for plate dimensions. The rigid side walls make the plate ideal for automation and the wide skirt accepts barcodes. Individual filter discs are encapsulated in the plate by a process that ensures 100% integrity of each well. The design of the nozzle prevents sample wicking and cross-contamination. Glass fibre filter plates can be used for a variety of applications, such as plasmid isolation, DNA purification, PCR clean-up or receptor/ligand binding assays. The low-binding hydrophilic PVDF membrane can be used for lysate clarification, protein kinase assays, or bead- or resin-based separation assays.
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Monomeric avidin agarose, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Monomeric avidin agarose is immobilised onto 4% beaded agarose for purifying biotinylated proteins, peptides and other molecules.
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