hMSC Adipogenesis Induction Medium

The hMSC adipogenesis induction medium is a sterile liquid culture medium for inducing adipogenic differentiation of human mesenchymal stem cells (hMSC). The medium is delivered as a basal medium and is suitable for culturing hMSC after adding the supplement kit components.

• Formulation is optimised for initial seeding of 6000 cells/cm² up to confluence (approx. 90%)
• Feeder-layer, matrix substrates, or other substances are not necessary
• Due to the possibility of reduced proliferative activity, we recommend to use the antibiotic supplement for freshly isolated cells only
• Product undergoes rigorous filtration and is tested for mycoplasma, endotoxin, and sterility to ensure its suitability for cell culture

After adding 50 ml FCS, 10 ml HEPES and 5 ml L-Glutamine and optional 3,5 ml antibiotics to the 500 ml hMSC adipogenesis induction medium basal the media can be stored in the dark at 2 to 8 °C for up to 4 weeks. After adding the induction factor Dexamethasone, Indomethacine, 3-Isobutyl-1-methyl-xanthine and insulin to the media, the media can be stored in the dark at 4 to 8 °C for up to 1 week.

Therefore, each aliquot contains enough volume to prepare 50 ml hMSC adipogenesis induction media only. To prepare 50 ml adipogenesis inductions media add 500 µl Dexamethasone, 500 µl Indomethacine, 500 µl 3-Isobutyl-1-methyl-xanthine, and 500 µl Insulin to 50 ml hMSC adipogenesis induction medium, basal (already supplemented with FCS, HEPES and L-Glutamine). Do not use the supplemented media for longer than one week.

Do not heat the medium over 37 °C or use uncontrollable sources of heat (e.g. microwave appliances). If only a part of the medium is to be used, remove this amount from the bottle and heat it. Do not freeze the medium. This can lead to high salt concentrations by freezing out pure water which will cause irreversible damage.

Storge: It is recommended to store this product at −80 °C, and caution should be taken to avoid subjecting it to repeated freeze/thaw cycles, as this can compromise its integrity and effectiveness. Proper storage conditions are essential for maintaining the stability and functionality of the product over time.