NucSpot®

NucSpot® nuclear stains are cell membrane-impermeant, nuclear-specific counterstains available in a variety of colors from green to near-infrared (near-IR).

• Nuclear-specific counterstains for fixed cells
• Selectively stains dead cells in live culture
• No wash required
• Options for cell cycle profiling by flow cytometry in fixed/permeabilised cells
• Color options for FITC, Cy®3, PE, Texas Red®, Cy®5, APC, Cy®5,5, and Cy®7 channels

NucSpot® stains have minimal fluorescence until they bind to DNA and can be used for no-wash nuclear staining. Unlike other nucleic acid dyes such as propidium iodide (PI), TOTO®, TO-PRO®, and similar dyes that stain both the nucleus and cytoplasm, NucSpot® nuclear stains selectively stain the nucleus in fixed and permeabilised cells without the need for RNase treatment.

NucSpot® nuclear stains also can be used for selective staining of dead cells in unfixed cell cultures for analysis by flow cytometry or fluorescence imaging. Several of the dyes can be continuously incubated with cells for multi-day imaging. NucSpot® 470 and NucSpot® far-red can be used for DNA content analysis of cell cycle by flow cytometry in fixed and permeabilised cells without requiring RNase treatment, unlike propidium iodide.