Affinity chromatography columns, GSTrap™ FF

GSTrap™ FF sind mit Glutathion Sepharose™ Fast Flow vorgepackte Säulen, die eine schnelle, praktische Reinigung von GST-markierten Proteinen ermöglichen.

• Milde Elutionsbedingungen erhalten die Antigenität und Funktion der Proteine
• Einfaches Scale-up durch Verbinden der Säulen in Serie
• Sepharose™ 4 Fast Flow bietet gute Flusseigenschaften
• Einfache Bedienung von GSTrap™ FF mit einer Spritze, einer Pumpe oder einer Chromatographiesystem wie ÄKTAdesign oder FPLC

GST-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione.

The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.

The resin, Glutathione Sepharose 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA design or FPLC System.