Experimentation kits, Water Quality Testing III: Multiplex PCR testing of water contaminants

EDVO953

Experimentation kits, Water Quality Testing III: Multiplex PCR testing of water contaminants

EDVO953

Bezeichnung:
Water Quality Testing III: Multiplex PCR testing of water contaminants
Environmentally Preferable:
VE:
1

Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.

For 5 lab groups
Set up 60 minutes; PCR 2 hours or overnight; electrophoresis 45 minutes

Also required: Automatic micropipettes (5 to 50 µl) and tips, waterbath, ice, balance, microcentrifuge, thermal cycler, electrophoresis apparatus, power supply, microwave or hot plate, UV transilluminator.

: Kit includes instructions, control DNA and primers, DNA ladder, nonpathogenic E. coli, B. subtilis, S. marcenscens, proteinase K, PCR beads, gel loading dye, agarose, electrophoresis buffer, InstaStain® Ethidium Bromide and FlashBlue™ stain.