Protein G Sepharose™ 4 Fast Flow

Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.

For antibody affinity chromatography
Fractionation of monoclonal antibodies without binding albumin
Recombinant protein G lacking albumin-binding region
Binding specificities that complement Protein A media
Binds a broad range of IgG species and subclasses
Cyanogen bromide activation coupling method
Multi-point attachment minimises ligand leakage
Used in a range of research applications

As member of the BioProcess media range, Protein G Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Protein G exhibit binding specificities that complement Protein A media and binds to the Fc region of IgG from a variety of mammalian species. Protein G Sepharose 4 Fast Flow may be used to isolate and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium.

pH-Bereich:
3 - 9
Matrix:
Cross-linked agarose, 4%, spherical
Ligand:
Recombinant protein G lacking albumin-binding region, produced in E. coli
Lagertemperatur:
2 - 8 °C
Ligand density:
2 mg Protein G/ml
Ligand coupling method:
Cyanogen bromide activation