Human AP1M1 ELISA Kit

Human AP1M1 ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of human AP1M1 in serum, plasma, tissue homogenates, and other biological fluids.

• Ready to use ELISA kit
• Detection range: 0,313 to 20 ng/ml
• Sensitivity: 0,188 ng/ml
• Sample type: Serum, plasma, tissue homogenates and other biological fluids
• Assay precision: Intra assay: CV <8%, Inter assay: CV <10%

Human AP1M1 ELISA kit (A76144) employs the competitive enzyme immunoassay technique for the quantitative measurement of human AP1M1 in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with AP1M1 antigen. During the incubation, AP1M1 present in the samples or standards competes with the fixed amount of immobilised AP1M1 for binding sites on the Biotinylated Anti-AP1M1 Antibody. The more AP1M1 present in a sample or standard, the less Biotinylated Anti-AP1M1 Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-AP1M1 Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of AP1M1 present in each sample or standard. The concentration of AP1M1 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

Ordering information: This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.