Serum-free neurophysiological basal medium for improved neuronal function.

Promote, rather than inhibit, neuronal activity and maturity in cultured primary or human pluripotent stem cell (hPSC)-derived neurons in a phenol red-free environment. BrainPhys™ serum-free neuronal basal medium is optimised to yield a higher proportion of synaptically active neurons by mimicking the central nervous system extracellular environment. Use BrainPhys™ without phenol red to culture primary neurons, differentiate and mature hPSC-derived neurons, record microelectrode array-based neuronal activity, live-fluorescent image neurons, and transdifferentiate somatic cells to neurons without hormonal signaling. To ensure cell survival in long-term serum-free culture, BrainPhys™ without phenol red must be combined with an appropriate serum-replacement supplement, such as NeuroCult™ SM1 neuronal supplement and/or N2 supplement-A.