CD8a (clone SK1) is a validated mouse monoclonal antibody that recognizes CD8a, a transmembrane glycoprotein and co-receptor found in T cells. This antibody derived from the CD8 alpha chain. The antibody is available conjugated to CF® Dyes and Astral™ tandem dyes. They are supplied in PBS, 0,1% BSA, 0,05% azide.

• Cell/tissue expression: Lymphocytes, T-cells
• Tumor expression: Leukemia/lymphoma
• SwissProt: P01732
• Unigene: 3003
• Positive control: Human peripheral blood mononuclear cells (PBMCs), CCRF-CEM or HL-60
• Ex/Em: 667/685 nm

CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4 /CD8-. Anaplastic large cell lymphoma is usually CD4 and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.

Type: Primary
Antigen: CD8a
Clonality: Monoclonal
Clone: SK1
Conjugation:CF®660C
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human