The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).

• Teaches recombinant protein expression
• Students learn about bacterial growth phases and inducible vectors

Also required: Shaking incubator, low speed centrifuge for 1,5 to 2 ml tubes, protein electrophoresis equipment, water bath or beaker and thermometer.