This experiment introduces a simple method to determine protein subunit molecular weights using horizontal electrophoresis. As the protein standards and "unknowns" are prestained, the separation of proteins can be observed during electrophoresis. Included in the experiment is protein-grade agarose, which provides an alternative to the use of polyacrylamide gels.

• For 4 lab groups
• Complete in 1 hour followed by overnight staining

Also required: Horizontal electrophoresis apparatus, power supply, white light visualisation system, 5 to 50 µl adjustable or 20 µl fixed volume micropipette, methanol, and glacial acetic acid.