For the capture and pulldown of nitrated proteins from cell and tissue lysates.

• Determine change in tyrosine nitration in treated versus non-treated samples
• Demonstrate target proteins are nitrated in vitro
• Identify and characterise nitrated proteins by Western blot and proteomic analysis

Cayman’s Nitrotyrosine IP Kit allows for the capture and pulldown of nitrated proteins from cell lysates using a sorbent coupled with a nitrotyrosine monoclonal antibody. The nitrated proteins can be eluted from the resin, loaded to an SDS-PAGE gel, and probed using the included Nitrotyrosine Polyclonal Antibody or a user supplied target specific antibody. A positive control sample assures that the assay is working.