Primary Human Glomerular Mesangial Cells

Our primary human cells offer unmatched consistency and a more biologically relevant tool than other cell types. Our cells are antibody-free and come from a deep inventory to help researchers with reproducibility and improved human insights.

• Highly proliferative and healthy (~85% viability)
• Antibody free and more biologically relevant
• Offered with high cell count (~1 million cells per vial)
• Well-cited in scientific journals
• Ideal for 2D and 3D cultures, microfluidic devices and co-cultures

Primary human glomerular mesangial cells (ACBRI127) were initiated by elutriation of dispase dissociated decapsulated glomeruli isolated from normal human cortical kidney tissue. This cell is antibody free. Each vial will initiate a 25 to 75 cm² cell culture. These primary cells are isolated, frozen and qualified using cell systems certified media and reagents which are specified for use in the research environment. Cell systems primary cells are exclusively from normal, site-specific human tissue specimen, and available in frozen vial or proliferating flask.

These cells were originated using cell systems complete serum free medium (MSPP-SF4Z0500), and subsequently grown and passaged in cell systems complete medium (4Z0-500). They are available at Passage 3 (<12 cumulative population doubling) cryopreserved in cell systems Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75 cm² flask. Each vial contains more than 1×106 cells.

Each vial of cells is shipped with vials of Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (human recombinant growth factors) at no additional cost.

Standard Tests:
HIV Serologic Test (donor level HIV AB EIA) -Negative
HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) - Negative
Hepatitis B (HBV) PCR Test (frozen cells) - Negative
Hepatitis C (HCV) PCR Test (frozen cells) - Negative
Mycoplasma spp. (frozen cells) (ATCC method by CLIA Licensed Clinical Lab) - Negative.

Characterization:
Inducible expression of CD 62E (E-Selectin) <2% positive by immunofluorescence
Cytoplasmic Vimentin Intermediate Filaments >98% positive by immunofluorescence
Cytoplasmic Alpha-Actinin Microfilaments >98% positive by immunofluorescence
Cytoplasmic Fibronectin >98% positive by immunofluorescence.

Caution: For research use only