SURE competent cells
Über diesen Artikel
SURE 2 competent cells are ideal for routine cloning of DNA with secondary structures.
- Lack components of the pathways that catalyse the rearrangement and deletion of nonstandard secondary and tertiary structures
- Allows for blue-white colour screening
Replicating eukaryotic DNA in prokaryotic cells can be problematic. Particular eukaryotic genes may contain inverted repeats or secondary structures, such as Z-DNA, that can be rearranged or deleted by E. coli DNA repair systems.
SURE competent cells are deficient in the E. coli genes involved in the rearrangement and deletion of DNA, thus improving cloning efficiencies of DNA containing irregular structures in prokaryotic cells. SURE cells are restriction minus (McrA-, McrCB-, McrF-, Mrr-, HsdR-) endonuclease (endA) deficient, and recombination (recB recJ) deficient.
: For research use only. Not for use in diagnostic procedures.
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Product Details & Documents
SURE 2 competent cells are ideal for routine cloning of DNA with secondary structures.
- Lack components of the pathways that catalyse the rearrangement and deletion of nonstandard secondary and tertiary structures
- Allows for blue-white colour screening
Replicating eukaryotic DNA in prokaryotic cells can be problematic. Particular eukaryotic genes may contain inverted repeats or secondary structures, such as Z-DNA, that can be rearranged or deleted by E. coli DNA repair systems.
SURE competent cells are deficient in the E. coli genes involved in the rearrangement and deletion of DNA, thus improving cloning efficiencies of DNA containing irregular structures in prokaryotic cells. SURE cells are restriction minus (McrA-, McrCB-, McrF-, Mrr-, HsdR-) endonuclease (endA) deficient, and recombination (recB recJ) deficient.
: For research use only. Not for use in diagnostic procedures.
Product Family Options
Produktinformationen
- BezeichnungEigenschaftPk InfoVerfügbarkeitPreis
- SURE electroporation-competent cellsFor cloning DNA with secondary structures, ≥1×10¹⁰ transformants/µg5×0,1 mlEinloggen für Verfügbarkeit1 * 5 STSFr. 454.00
- SURE competent cellsFor routine cloning of DNA with secondary structures, ≥5×10⁸ transformants/µg5×0,2 mlEinloggen für Verfügbarkeit1 * 1 mLSFr. 433.00
- SURE 2 supercompetent cellsHigh-efficiency derivative for cloning DNA with secondary structures, >1×10⁹ transformants/µg10×0,1 mlEinloggen für Verfügbarkeit1 * 1 mLSFr. 442.00
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