Human Recombinant PDGF-AB (from E. coli)

Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-α, TGF-β and EGF signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells.

High quality
Low endotoxin
Affordable

PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-α and PDGFR-β.

: Certificate of Analysis (COA) is supplied with each product shipped detailing purity, formulation, biological activity, endotoxin levels, reconstitution instructions and recommended storage conditions.

: For research use only.

Konjugation:
Unconjugated
Protein-Funktion:
Cytokine
Protein/Peptid-Typ:
Rekombinant
Ursprung:
E. coli
Spezies:
Human
Lagerbedingungen:
−20 °C
Endotoxingehalt:
Endotoxin LAL (EU/µg) ≤0.1
Biologische Aktivität:
Bioactivity assay: NR6R-3T3 cell proliferation, ED50 ≤20 ng/ml, bioactivity ≥5,0×10⁴ units/mg
Gen-ID:
P04085/P01127
Reconstitution instructions:
Sterile water at 0,1 mg/ml
Frei von Endotoxinen:
N
Frei von Trägern:
Y
Frei von Proteasen:
N
Tierfrei:
N
Proteinsynonym:
ODGF|GDGF
UniProtKB:
P04085/P01127
Protein/Peptid-Name:
PDGF-AB
Purity:
≥95%
Molecular weight:
Alpha: 14,4 kDa, beta: 12,4 kDa, total 26,8
Sequence:
Alpha chain: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT Beta chain: MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT
Endotoxinspiegel:
Low
Zusammensetzung:
0,1% Trifluoroacetic Acid (TFA)
Frei von Nukleasen:
N
Sorte:
RUO
Transporttemperatur:
RT
Validierte Anwendungen:
Bioassay