Proteasome Purification Kit

Proteasome Purification Kit is a high integrity kit for the isolation and purification of proteasome complexes from biological samples such as cell lysates, tissue extracts, plasma, serum, confirmed by Western blot analysis.

• Isolation of wild type proteasome complexes from biological samples (cell lysates, tissue extracts, plasma, serum), confirmed by Western blot analysis
• Analysis of captured proteasome complex proteins by proteomic methods, for example to determine complex composition and subunit post-translational modification
• Investigation of variations in proteasome complexes in abnormal cell lines/tissues
• Comparison of proteasome complexes isolated from biological samples associated with a particular disease/illness with samples from healthy controls

This kit provides a gentle and rapid means to purify proteasome complexes from biological samples using an agarose immobilised 20S subunit specific proteasome antibody, without the need for genetic modification of the expressed proteasome. Captured proteasomes are eluted under denaturing conditions prior to analysis by Western blotting or characterisation by proteomic methods.

 : Kit includes: Proteasome purification matrix, control lysate, proteasome 20S β5, pAb.