The iodoTMTsixplex™ labelling reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry.

• Specificaly reacts only with sulfhydryl groups (reduced, unmodified cysteine residues)
• Labelled proteins and peptides are not susceptible to reducing agents
• Flexible for both duplex isotopic and sixplex isobaric quantitation of protein
• Can be used to study cysteine modifications (eg. di-sulphide bridges, S-nitrosylation)

The iodoTMT™ reagents are sets of isobaric (mass and structure) isomers that are iodoacetyl-activated for covalent, irreversible labeling of sulphhydryl (—SH) groups. They can be differentiated by mass spectrometry (MS), enabling quantitation of the relative abundance of cysteine modifications, such as S-nitrosylation, oxidation and disulfide bonds, in cultured cells grown or treated with different conditions.