Marfey's Reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide) reacts with primary amines to enable the quick and easy separation and quantitation of optical isomers of amino acids by reverse-phase chromatography.

• For precolumn derivatisation of primary amines for detection by UV absorbance (λmax = 340nm)
• Provides nanomole sensitivity in typical HPLC applications with amino acids
• Reaction products enable chiral separation of D- and L-amino acids
• Following reverse phase HPLC, derivatives have an extinction coefficient of 3×10⁴ M^-1 cm^-1