Classic IP kit, Pierce™

The Pierce™ Classic IP Kit uses protein A/G agarose beads, microcentrifuge spin columns and buffers to perform traditional immunoprecipitation assays.

• Spin columns eliminate resin loss and provide efficient separation of solutions
• Immunoprecipitate (IP) in less than one hour
• Recover antigen without harsh detergents or reducing agents
• Purify target protein from crude lysate in four simple steps

This kit involves formation of antibody:antigen complexes in a sample solution and then capture of that complex to an IgG-binding protein that is covalently bound to beaded agarose resin. The microcentrifuge spin column format helps to ensure effective washing and separation of samples from the beaded agarose affinity resin.

 : The kit includes protein A/G plus agarose, 0,55 ml; IP lysis/wash buffer, 2×50 ml; conditioning buffer (100X), 5 ml; tris-buffered saline (20X), 25 ml; elution buffer, 50 ml; lane marker sample buffer (5X), 5 ml; control agarose resin, 2 ml; spin columns and collection tubes.