The Biosensis NGF Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of rat NGF in less than 3 hours in cell culture supernatants, serum, and brain extracts only if used as directed.

This NGF ELISA kit is designed to measure rat NGF and thus employs a recombinant rat NGF protein. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and mouse! Guinea pig NGF has been quantified in serum using this ELISA kit using rat NGF protein as calibrator, as it shows largest sequence homology based on amino acid sequence among rodent and human NGF proteins. This ELISA kit does not detect the full-length proNGF protein.

The purpose of this kit is the in vitro quantitative determination of NGF in samples such as serum, plasma, cell culture supernatants and brain extracts.

The assay antibodies preferentially detect the mature form of NGF, as shown by data comparing mature mouse NGF and mouse proNGF in the Mouse NGF ELISA kit. Cross-reaction of full-length proNGF was < 0,1% when assayed at 25 ng/ml in assay buffer, and not detectable when assayed across the mature NGF calibration range.

This kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of NGF present in samples and protein standards.

Please refer to the kit protocol for specific use instructions for each substrate application, in particular serum samples.